TY - JOUR
T1 - Characterization of O,P′-DDT-stimulated contraction frequency in rat uterus in Vitro
AU - Juberg, Daland R.
AU - Webb, R. Clinton
AU - Loch-caruso, Rita
N1 - Funding Information:
We thank Dr. Craig Harris for uterine tissue. This research was conducted as partial fulfillment of D.R.J.‘s doctoral dissertation, and was supported, in part, with predoctoral training grants to D.R.J. (NIH HD07048 and NIH GM07767) and research grants to R.L.C. (NIH ES04424) and R.C.W. (NIH HL27020). Additional support was provided by the Laboratory Animal and Data Analysis Cores of the P30 Center for the Study of Reproduction (NIH HD18258).
PY - 1991/10
Y1 - 1991/10
N2 - Characterization of O,P′-DDT-Stimulated Contraction Frequency in Rat Uterus in Vitro. JUBERG, D. R., WEBB, R. C., AND LOCH-CARUSO, R. (1991). Fundam. Appl. Toxicol. 17, 543-549. Exposure to organochlorine pesticides, including DDT, has previously been associated with premature birth. Using an improved protocol to characterize dose and time dependent responses, the present report extends a preliminary finding by this laboratory that O,P′-DDT directly stimulates uterine contractility. Contraction frequency was determined in longitudinal uterine strips from pregnant rats under isometric force conditions. Following equilibration, the uterine strips were monitored for a 1-hr baseline period, then treated with O,P′-DDT or ethanol (solvent control) for 3 hr, followed by 3 hr without test substance. During exposure to 100 μM O,P′-DDT, the frequency of contraction significantly increased by 66% relative to matched controls. After removal of O,P′-DDT from the medium, the frequency of contraction continued to increase in uterine strips exposed to 50 and 100 μM O,P′-DDT. A dose effect was clearly observed during the post-treatment period, with 50 and 100 μM O,P′-DDT significantly increasing contraction frequency by 39 and 104% relative to controls. No significant differences in contraction frequency were observed with 10 μM O,P′-DDT during any test period. These data show that O,P′-DDT directly stimulated isometric contractions in rat uterine strips.
AB - Characterization of O,P′-DDT-Stimulated Contraction Frequency in Rat Uterus in Vitro. JUBERG, D. R., WEBB, R. C., AND LOCH-CARUSO, R. (1991). Fundam. Appl. Toxicol. 17, 543-549. Exposure to organochlorine pesticides, including DDT, has previously been associated with premature birth. Using an improved protocol to characterize dose and time dependent responses, the present report extends a preliminary finding by this laboratory that O,P′-DDT directly stimulates uterine contractility. Contraction frequency was determined in longitudinal uterine strips from pregnant rats under isometric force conditions. Following equilibration, the uterine strips were monitored for a 1-hr baseline period, then treated with O,P′-DDT or ethanol (solvent control) for 3 hr, followed by 3 hr without test substance. During exposure to 100 μM O,P′-DDT, the frequency of contraction significantly increased by 66% relative to matched controls. After removal of O,P′-DDT from the medium, the frequency of contraction continued to increase in uterine strips exposed to 50 and 100 μM O,P′-DDT. A dose effect was clearly observed during the post-treatment period, with 50 and 100 μM O,P′-DDT significantly increasing contraction frequency by 39 and 104% relative to controls. No significant differences in contraction frequency were observed with 10 μM O,P′-DDT during any test period. These data show that O,P′-DDT directly stimulated isometric contractions in rat uterine strips.
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U2 - 10.1093/toxsci/17.3.543
DO - 10.1093/toxsci/17.3.543
M3 - Article
C2 - 1794656
AN - SCOPUS:77957178339
SN - 1096-6080
VL - 17
SP - 543
EP - 549
JO - Toxicological Sciences
JF - Toxicological Sciences
IS - 3
ER -