Characterization of PAF-AH Ib1 in NOD mice: PAF-AH may not be a candidate gene of the diabetes susceptibility Idd4.1 locus

Qing Sheng Mi; Li Zhou; Marsha Grattan; Zai Zhao Wang; M. Sivilotti; Jin-Xiong She; Terry L. Delovitch

doi:10.1196/annals.1375.022

Characterization of PAF-AH Ib1 in NOD mice: PAF-AH may not be a candidate gene of the diabetes susceptibility Idd4.1 locus

Qing Sheng Mi
, Li Zhou
, Marsha Grattan
, Zai Zhao Wang
, M. Sivilotti
, Jin-Xiong She
, Terry L. Delovitch

Obstetrics and Gynecology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We recently mapped Idd4 to a 5.2 cM interval on chromosome 11 with two subloci, Idd4.1 and Idd4.2, in nonobese diabetic (NOD) mice. Based on the localization of platelet-activating factor acetylhydrolase Ib1 (PAF-AHIb1) and the decreased activity of PAF-AH in type 1 diabetes (T1D) patients, we hypothesized that PAF-AHIb1 in Idd4.1 is a candidate gene. The PAF-AHIb1 gene in NOD mice was cloned and sequenced, and its expression and function were studied. No polymorphisms were detected in PAF-AHIb1 cDNA between NOD and B6 mice. The expression of PAF-AH Ib1 at the mRNA and protein levels was found to be similar in different tissues between NOD and B6 mice. PAF-AH activity does not differ in the pancreatic islets or spleen between NOD and B6 mice. Our findings suggest that PAF-AH Ib1 may not be a diabetes-susceptibility gene in the Idd4.1 sublocus.

Original language	English (US)
Title of host publication	Immunology of Diabetes IV
Subtitle of host publication	Progress in Our Understanding
Publisher	Blackwell Publishing Inc.
Pages	147-152
Number of pages	6
ISBN (Print)	1573316415, 9781573316415
DOIs	https://doi.org/10.1196/annals.1375.022
State	Published - Oct 2006

Publication series

Name	Annals of the New York Academy of Sciences
Volume	1079
ISSN (Print)	0077-8923
ISSN (Electronic)	1749-6632

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Diabetes
Genetic susceptibility
Idd4
Nonobese diabetic mice
Platelet-activating factor acetylhydrolase Ib1 gene

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
History and Philosophy of Science

Access to Document

10.1196/annals.1375.022

Cite this

Mi, Q. S., Zhou, L., Grattan, M., Wang, Z. Z., Sivilotti, M., She, J.-X., & Delovitch, T. L. (2006). Characterization of PAF-AH Ib1 in NOD mice: PAF-AH may not be a candidate gene of the diabetes susceptibility Idd4.1 locus. In Immunology of Diabetes IV: Progress in Our Understanding (pp. 147-152). (Annals of the New York Academy of Sciences; Vol. 1079). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1375.022

Characterization of PAF-AH Ib1 in NOD mice: PAF-AH may not be a candidate gene of the diabetes susceptibility Idd4.1 locus. / Mi, Qing Sheng; Zhou, Li; Grattan, Marsha et al.
Immunology of Diabetes IV: Progress in Our Understanding. Blackwell Publishing Inc., 2006. p. 147-152 (Annals of the New York Academy of Sciences; Vol. 1079).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Mi, QS, Zhou, L, Grattan, M, Wang, ZZ, Sivilotti, M, She, J-X & Delovitch, TL 2006, Characterization of PAF-AH Ib1 in NOD mice: PAF-AH may not be a candidate gene of the diabetes susceptibility Idd4.1 locus. in Immunology of Diabetes IV: Progress in Our Understanding. Annals of the New York Academy of Sciences, vol. 1079, Blackwell Publishing Inc., pp. 147-152. https://doi.org/10.1196/annals.1375.022

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abstract = "We recently mapped Idd4 to a 5.2 cM interval on chromosome 11 with two subloci, Idd4.1 and Idd4.2, in nonobese diabetic (NOD) mice. Based on the localization of platelet-activating factor acetylhydrolase Ib1 (PAF-AHIb1) and the decreased activity of PAF-AH in type 1 diabetes (T1D) patients, we hypothesized that PAF-AHIb1 in Idd4.1 is a candidate gene. The PAF-AHIb1 gene in NOD mice was cloned and sequenced, and its expression and function were studied. No polymorphisms were detected in PAF-AHIb1 cDNA between NOD and B6 mice. The expression of PAF-AH Ib1 at the mRNA and protein levels was found to be similar in different tissues between NOD and B6 mice. PAF-AH activity does not differ in the pancreatic islets or spleen between NOD and B6 mice. Our findings suggest that PAF-AH Ib1 may not be a diabetes-susceptibility gene in the Idd4.1 sublocus.",

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AB - We recently mapped Idd4 to a 5.2 cM interval on chromosome 11 with two subloci, Idd4.1 and Idd4.2, in nonobese diabetic (NOD) mice. Based on the localization of platelet-activating factor acetylhydrolase Ib1 (PAF-AHIb1) and the decreased activity of PAF-AH in type 1 diabetes (T1D) patients, we hypothesized that PAF-AHIb1 in Idd4.1 is a candidate gene. The PAF-AHIb1 gene in NOD mice was cloned and sequenced, and its expression and function were studied. No polymorphisms were detected in PAF-AHIb1 cDNA between NOD and B6 mice. The expression of PAF-AH Ib1 at the mRNA and protein levels was found to be similar in different tissues between NOD and B6 mice. PAF-AH activity does not differ in the pancreatic islets or spleen between NOD and B6 mice. Our findings suggest that PAF-AH Ib1 may not be a diabetes-susceptibility gene in the Idd4.1 sublocus.

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Characterization of PAF-AH Ib1 in NOD mice: PAF-AH may not be a candidate gene of the diabetes susceptibility Idd4.1 locus

Abstract

Publication series

UN SDGs

Keywords

ASJC Scopus subject areas

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