TY - JOUR
T1 - Characterization of the antioxidant activity of aglycone and glycosylated derivatives of hesperetin
T2 - An in vitro and in vivo study
AU - De Souza, Verônica Trícoli
AU - De Franco, Élida Paula Dini
AU - De Araújo, Maria Elisa Melo Branco
AU - Messias, Márcia Cristina Fernandes
AU - Priviero, Fernanda Bruschi Marinho
AU - Frankland Sawaya, Alexandra C.H.
AU - De Oliveira Carvalho, Patricia
N1 - Publisher Copyright:
Copyright © 2015 John Wiley & Sons, Ltd.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - The flavonoids are mainly present in Citrus fruits as their glycosyl derivatives. This study was conducted comparing in vitro xanthine oxidase inhibitory activity of the aglycone hesperetin and its glycosylated forms (hesperidin and G-hesperidin) and their effects on the plasma lipid profile and the oxidative-antioxidative system (TBARS and antioxidant enzymes) in rats. The concentrations of the major conjugated metabolites in rat plasma after oral administration of these compounds were also determined. Wistar male rats were randomly assigned to three groups (n = 6) supplemented for 30 days with 1 mmol/kg body mass of hesperetin, hesperidin or G-hesperidin. Hesperetin was a stronger xanthine oxidase inhibitor (IC50 = 53 μM and Ki = 17.3 μM) than the glycosylate derivatives. Supplementation with the three compounds led to a lower (more favorable) atherogenic index, and an antioxidant preventive effect from the increase of hepatic superoxide dismutase was observed associated to HT supplementation, possibly because of the higher level of hesperetin-glucuronide in rat plasma.
AB - The flavonoids are mainly present in Citrus fruits as their glycosyl derivatives. This study was conducted comparing in vitro xanthine oxidase inhibitory activity of the aglycone hesperetin and its glycosylated forms (hesperidin and G-hesperidin) and their effects on the plasma lipid profile and the oxidative-antioxidative system (TBARS and antioxidant enzymes) in rats. The concentrations of the major conjugated metabolites in rat plasma after oral administration of these compounds were also determined. Wistar male rats were randomly assigned to three groups (n = 6) supplemented for 30 days with 1 mmol/kg body mass of hesperetin, hesperidin or G-hesperidin. Hesperetin was a stronger xanthine oxidase inhibitor (IC50 = 53 μM and Ki = 17.3 μM) than the glycosylate derivatives. Supplementation with the three compounds led to a lower (more favorable) atherogenic index, and an antioxidant preventive effect from the increase of hepatic superoxide dismutase was observed associated to HT supplementation, possibly because of the higher level of hesperetin-glucuronide in rat plasma.
KW - bioavailability
KW - hesperetin
KW - lipid profile
KW - oxidative-antioxidative system
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U2 - 10.1002/jmr.2509
DO - 10.1002/jmr.2509
M3 - Article
C2 - 26370929
AN - SCOPUS:84955198496
SN - 0952-3499
VL - 29
SP - 80
EP - 87
JO - Journal of Molecular Recognition
JF - Journal of Molecular Recognition
IS - 2
ER -