Characterization of thermophilic bacteria using surface-enhanced Raman scattering

Mustafa Çulha, Ahmet Adigüzel, M. Müge Yazici, Mehmet Kahraman, Fikrettin Şahin, Medine Güllüce

Research output: Contribution to journalArticlepeer-review

58 Scopus citations


Surface-enhanced Raman scattering (SERS) can provide molecular-level information about the molecules and molecular structures in the vicinity of nanostructured noble metal surfaces such as gold and silver. The three thermophilic bacteria Bacillus licheniformis, Geobacillus stearothermophilus, and Geobacillus pallidus, a Gram-negative bacterium E. coli, and a Gram-positive bacterium B. megaterium are comparatively characterized using SERS. The SERS spectra of thermophilic bacteria are similar, while they show significant differences compared to E. coli and B. megaterium. The findings indicate that a higher number of thiol residues and possible S-S bridges are present in the cell wall structure of thermophilic bacteria, providing their stability at elevated temperatures. Incubating the thermophilic bacteria with colloidal silver suspension at longer times improved the bacteria-silver nanoparticle interaction kinetics, while increased temperature does not have a pronounced effect on spectral features. A tentative assignment of the SERS bands was attempted for thermophilic bacteria. The results indicate that SERS can be a useful tool to study bacterial cell wall molecular differences.

Original languageEnglish (US)
Pages (from-to)1226-1232
Number of pages7
JournalApplied Spectroscopy
Issue number11
StatePublished - Nov 2008
Externally publishedYes


  • Bacteria
  • Cell walls
  • SERS
  • Silver nanoparticles
  • Surface-enhanced raman spectroscopy
  • Thermophilic bacteria

ASJC Scopus subject areas

  • Instrumentation
  • Spectroscopy


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