Abstract
An increasing number of transcription factors have been shown to activate DNA replication. However, the underlying mechanism remains to be elucidated. Here it is shown that when tethered to a cellular replication origin, the acidic transcriptional activation domain of the breast cancer protein BRCA1 alters the local chromatin structure and stimulates chromosomal DNA replication. Cancer-predisposing mutations in BRCA1 that abolish transcriptional activation also prevent chromatin remodeling and activation of replication. Chromatin remodeling occurs even in the absence of a functional replication origin. Thus, increasing chromatin accessibility may be an important mechanism used by transcription factors to facilitate multiple nuclear processes.
Original language | English (US) |
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Pages (from-to) | 637-642 |
Number of pages | 6 |
Journal | Genes and Development |
Volume | 13 |
Issue number | 6 |
DOIs | |
State | Published - Mar 15 1999 |
Externally published | Yes |
Keywords
- ARS1
- BRCA1
- Chromatin remodeling
- Replication
- Transcription factors
- Yeast
ASJC Scopus subject areas
- Genetics
- Developmental Biology