Chromophore-assisted laser inactivation in cell biology

Ken Jacobson; Zenon Rajfur; Eric Vitriol; Klaus Hahn

doi:10.1016/j.tcb.2008.07.001

Chromophore-assisted laser inactivation in cell biology

Ken Jacobson, Zenon Rajfur, Eric Vitriol, Klaus Hahn

Research output: Contribution to journal › Review article › peer-review

110 Scopus citations

Abstract

Chromophore-assisted laser inactivation (CALI) is a technique whereby engineered proteins and dye molecules that produce substantial amounts of reactive oxygen species upon absorption of light are used to perturb biological systems in a spatially and temporally defined manner. CALI is an important complement to conventional genetic and pharmacological manipulations. In this review, we examine the applications of CALI to cell biology and discuss the underlying photochemical mechanisms that mediate this powerful technique.

Original language	English (US)
Pages (from-to)	443-450
Number of pages	8
Journal	Trends in Cell Biology
Volume	18
Issue number	9
DOIs	https://doi.org/10.1016/j.tcb.2008.07.001
State	Published - Sep 2008
Externally published	Yes

ASJC Scopus subject areas

Cell Biology

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10.1016/j.tcb.2008.07.001

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title = "Chromophore-assisted laser inactivation in cell biology",

abstract = "Chromophore-assisted laser inactivation (CALI) is a technique whereby engineered proteins and dye molecules that produce substantial amounts of reactive oxygen species upon absorption of light are used to perturb biological systems in a spatially and temporally defined manner. CALI is an important complement to conventional genetic and pharmacological manipulations. In this review, we examine the applications of CALI to cell biology and discuss the underlying photochemical mechanisms that mediate this powerful technique.",

author = "Ken Jacobson and Zenon Rajfur and Eric Vitriol and Klaus Hahn",

year = "2008",

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doi = "10.1016/j.tcb.2008.07.001",

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AU - Jacobson, Ken

AU - Rajfur, Zenon

AU - Vitriol, Eric

AU - Hahn, Klaus

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N2 - Chromophore-assisted laser inactivation (CALI) is a technique whereby engineered proteins and dye molecules that produce substantial amounts of reactive oxygen species upon absorption of light are used to perturb biological systems in a spatially and temporally defined manner. CALI is an important complement to conventional genetic and pharmacological manipulations. In this review, we examine the applications of CALI to cell biology and discuss the underlying photochemical mechanisms that mediate this powerful technique.

AB - Chromophore-assisted laser inactivation (CALI) is a technique whereby engineered proteins and dye molecules that produce substantial amounts of reactive oxygen species upon absorption of light are used to perturb biological systems in a spatially and temporally defined manner. CALI is an important complement to conventional genetic and pharmacological manipulations. In this review, we examine the applications of CALI to cell biology and discuss the underlying photochemical mechanisms that mediate this powerful technique.

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DO - 10.1016/j.tcb.2008.07.001

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Chromophore-assisted laser inactivation in cell biology

Abstract

ASJC Scopus subject areas

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