Chronic ANG II infusion induces sex-specific increases in renal T cells in Sprague-Dawley rats

Margaret A. Zimmerman, Babak Baban, Ashlee J. Tipton, Paul M. O’Connor, Jennifer C. Sullivan

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


Recent studies suggest that sex of the animal and T cell impact ANG II hypertension in Rag_/_ mice, with females being protected relative to males. This study tested the hypothesis that ANG II results in greater increases in proinflammatory T cells and cytokines in males than in females. Male and female Sprague-Dawley (SD) rats, aged 12 wk, were treated with vehicle or ANG II (200 ng·kg-1·min-1) for 2 wk. Renal CD4+ T cells and Tregs were comparable between vehicletreated males and females, although males expressed more Th17 and IL-17+ T cells and fewer IL-10+ T cells than females. ANG II resulted in greater increases in CD4+ T cells, Th17 cells, and IL-17+cells in males; Tregs increased only in females. We previously showed that ANG (1–7) antagonizes ANG II-induced increases in blood pressure in females and ANG (1–7) has been suggested to be anti-inflammatory. Renal ANG (1–7) levels were greater in female SD at baseline and following ANG II infusion. Additional rats were treated with ANG II plus the ANG (1–7)-mas receptor antagonist A-779 (48μg·kg-1·h-1) to test the hypothesis that greater ANG (1–7) in females results in more Tregs relative to males. Inhibition of ANG (1–7) did not alter renal T cells in either sex. In conclusion, ANG II induces a sex-specific effect on the renal T cell profile. Males have greater increases in proinflammatory T cells, and females have greater increases in anti-inflammatory Tregs; however, sex differences in the renal T cell profile are not mediated by ANG (1–7).

Original languageEnglish (US)
Pages (from-to)F706-F712
JournalAmerican Journal of Physiology - Renal Physiology
Issue number7
StatePublished - 2015


  • ANG (1–7)
  • Adaptive immune system
  • Cytokines
  • Gender
  • Sex differences
  • Sprague-Dawley

ASJC Scopus subject areas

  • Physiology
  • Urology


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