TY - JOUR
T1 - Chronic ANG II infusion induces sex-specific increases in renal T cells in Sprague-Dawley rats
AU - Zimmerman, Margaret A.
AU - Baban, Babak
AU - Tipton, Ashlee J.
AU - O’Connor, Paul M.
AU - Sullivan, Jennifer C.
N1 - Publisher Copyright:
© 2015 the American Physiological Society.
PY - 2015
Y1 - 2015
N2 - Recent studies suggest that sex of the animal and T cell impact ANG II hypertension in Rag_/_ mice, with females being protected relative to males. This study tested the hypothesis that ANG II results in greater increases in proinflammatory T cells and cytokines in males than in females. Male and female Sprague-Dawley (SD) rats, aged 12 wk, were treated with vehicle or ANG II (200 ng·kg-1·min-1) for 2 wk. Renal CD4+ T cells and Tregs were comparable between vehicletreated males and females, although males expressed more Th17 and IL-17+ T cells and fewer IL-10+ T cells than females. ANG II resulted in greater increases in CD4+ T cells, Th17 cells, and IL-17+cells in males; Tregs increased only in females. We previously showed that ANG (1–7) antagonizes ANG II-induced increases in blood pressure in females and ANG (1–7) has been suggested to be anti-inflammatory. Renal ANG (1–7) levels were greater in female SD at baseline and following ANG II infusion. Additional rats were treated with ANG II plus the ANG (1–7)-mas receptor antagonist A-779 (48μg·kg-1·h-1) to test the hypothesis that greater ANG (1–7) in females results in more Tregs relative to males. Inhibition of ANG (1–7) did not alter renal T cells in either sex. In conclusion, ANG II induces a sex-specific effect on the renal T cell profile. Males have greater increases in proinflammatory T cells, and females have greater increases in anti-inflammatory Tregs; however, sex differences in the renal T cell profile are not mediated by ANG (1–7).
AB - Recent studies suggest that sex of the animal and T cell impact ANG II hypertension in Rag_/_ mice, with females being protected relative to males. This study tested the hypothesis that ANG II results in greater increases in proinflammatory T cells and cytokines in males than in females. Male and female Sprague-Dawley (SD) rats, aged 12 wk, were treated with vehicle or ANG II (200 ng·kg-1·min-1) for 2 wk. Renal CD4+ T cells and Tregs were comparable between vehicletreated males and females, although males expressed more Th17 and IL-17+ T cells and fewer IL-10+ T cells than females. ANG II resulted in greater increases in CD4+ T cells, Th17 cells, and IL-17+cells in males; Tregs increased only in females. We previously showed that ANG (1–7) antagonizes ANG II-induced increases in blood pressure in females and ANG (1–7) has been suggested to be anti-inflammatory. Renal ANG (1–7) levels were greater in female SD at baseline and following ANG II infusion. Additional rats were treated with ANG II plus the ANG (1–7)-mas receptor antagonist A-779 (48μg·kg-1·h-1) to test the hypothesis that greater ANG (1–7) in females results in more Tregs relative to males. Inhibition of ANG (1–7) did not alter renal T cells in either sex. In conclusion, ANG II induces a sex-specific effect on the renal T cell profile. Males have greater increases in proinflammatory T cells, and females have greater increases in anti-inflammatory Tregs; however, sex differences in the renal T cell profile are not mediated by ANG (1–7).
KW - ANG (1–7)
KW - Adaptive immune system
KW - Cytokines
KW - Gender
KW - Sex differences
KW - Sprague-Dawley
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U2 - 10.1152/ajprenal.00446.2014
DO - 10.1152/ajprenal.00446.2014
M3 - Article
C2 - 25503730
AN - SCOPUS:84965189505
SN - 0363-6127
VL - 308
SP - F706-F712
JO - American Journal of Physiology - Renal Physiology
JF - American Journal of Physiology - Renal Physiology
IS - 7
ER -