Abstract
Objective and design: The hypothesis that aspiration of gastric fluid drives the anti-ovalbumin response toward a Th2 reaction even in animals not prone to Th2 responses was evaluated. Subjects: Forty-eight male C57BL/6 mice were used. Methods: Mice were sensitized and challenged with ovalbumin starting 5 weeks prior to the initiation of weekly aspirations of either gastric fluid or normal saline as a control. Weekly aspiration continued during the course of exposure to ovalbumin. Treatment: Aspiration consisted of 50 μl of gastric fluid with 50 μl of 0.9 % normal saline used as a control. Antigen exposure consisted of sensitization to ovalbumin via intraperitoneal injection on days 0 and 14 and challenge on day 21 with aerosolized antigen for 30 min. Results: No evidence of a shift toward a Th2 response as a result of gastric fluid aspiration was seen in the Th1-prone strain utilized, although a profound down-regulation of a broad array of T cell-associated cytokines and chemokines and up-regulation of macrophage-associated markers was observed as a result of aspiration. Conclusions: These data provide support for the hypothesis that the clinical association between asthma and gastroesophageal reflux disease (GERD) does not involve an exacerbation of asthma by GERD-associated aspiration of gastric fluid, but may cause immune reactions unrelated to the asthma pathology.
Original language | English (US) |
---|---|
Pages (from-to) | 863-873 |
Number of pages | 11 |
Journal | Inflammation Research |
Volume | 61 |
Issue number | 8 |
DOIs | |
State | Published - Aug 2012 |
Keywords
- Adaptive immunity
- Aspiration
- Asthma
- Gastroesophageal reflux
- Innate immunity
ASJC Scopus subject areas
- Immunology
- Pharmacology