TY - JOUR
T1 - Class II transactivator knockdown limits major histocompatibility complex II expression, diminishes immune rejection, and improves survival of allogeneic bone marrow stem cells in the infarcted heart
AU - Huang, Xi Ping
AU - Ludke, Ana
AU - Dhingra, Sanjiv
AU - Guo, Jian
AU - Sun, Zhuo
AU - Zhang, Li
AU - Weisel, Richard D.
AU - Li, Ren Ke
N1 - Funding Information:
This study was supported by the Canadian Institutes of Health Research (Foundation Grant 332652 to R.-K.L.). A.L. is supported by a Heart and Stroke Foundation of Canada Fellowship Award. R.-K.L. holds a Canada Research Chair in Cardiac Regeneration. Author contributions: X.-P. Huang and A. Ludke designed and performed research, analyzed data and wrote the paper; S. Dhingra, J. Guo and Z. Sun performed research; L. Zhang contributed to data analysis and interpretation; and R. D. Weisel and R.-K. Li designed research and wrote the paper. The authors declare no conflicts of interest.
Publisher Copyright:
© FASEB.
PY - 2016/9
Y1 - 2016/9
N2 - This study was performed to investigate how to overcome immunorejection associated with allogeneic stem cell therapy in the infarcted heart. Allogeneic bone marrow mesenchymal stem cell (MSC) differentiation increases major histocompatibility complex II (MHC II) expression, inducing transition from immunoprivileged to immunogenic phenotype. MHC II expression is regulated by the class II transactivator (CIITA). We isolated and characterizedmouse and humanMSCs and knocked down CIITA expression.Wild-type (WT) or CIITA-knockout (CIITA-)mouseMSCs were implanted into infarcted mousemyocardia, and recipient allo-antibody formation, cell survival, and cardiac functionweremeasured.WTmouse and humanMSCs thatweremyogenically differentiated showed increased MHC II and CIITA expression. Differentiated CIITA- MSCs lacked MHC II induction and showed reduced cytotoxicity in allogeneic leukocyte coculture. Differentiation of humanMSCs increased MHC II expression, which resulted in cytotoxicity in allogeneic leukocyte coculture and was prevented by CIITA small interfering RNA. In contrast to WT MSCs, CIITA- MSCs did not initiate recipient allo-antibody formation and instead survived in the injured myocardium and significantly improved ventricular function. Decreasing CIITA expression in allogeneic MSCs abolished MHC II induction during myogenic differentiation and prevented immunorejection of these cells from the infarcted myocardium, which enhanced beneficial functional effects of MSC implantation onmyocardial repair.-Huang, X.-P., Ludke, A., Dhingra, S., Guo, J., Sun, Z., Zhang, L.,Weisel, R.D., Li, R.-K.Class II transactivator knockdown limitsmajor histocompatibility complex II expression, diminishes immune rejection, and improves survival of allogeneic bone marrow stem cells in the infarcted heart.
AB - This study was performed to investigate how to overcome immunorejection associated with allogeneic stem cell therapy in the infarcted heart. Allogeneic bone marrow mesenchymal stem cell (MSC) differentiation increases major histocompatibility complex II (MHC II) expression, inducing transition from immunoprivileged to immunogenic phenotype. MHC II expression is regulated by the class II transactivator (CIITA). We isolated and characterizedmouse and humanMSCs and knocked down CIITA expression.Wild-type (WT) or CIITA-knockout (CIITA-)mouseMSCs were implanted into infarcted mousemyocardia, and recipient allo-antibody formation, cell survival, and cardiac functionweremeasured.WTmouse and humanMSCs thatweremyogenically differentiated showed increased MHC II and CIITA expression. Differentiated CIITA- MSCs lacked MHC II induction and showed reduced cytotoxicity in allogeneic leukocyte coculture. Differentiation of humanMSCs increased MHC II expression, which resulted in cytotoxicity in allogeneic leukocyte coculture and was prevented by CIITA small interfering RNA. In contrast to WT MSCs, CIITA- MSCs did not initiate recipient allo-antibody formation and instead survived in the injured myocardium and significantly improved ventricular function. Decreasing CIITA expression in allogeneic MSCs abolished MHC II induction during myogenic differentiation and prevented immunorejection of these cells from the infarcted myocardium, which enhanced beneficial functional effects of MSC implantation onmyocardial repair.-Huang, X.-P., Ludke, A., Dhingra, S., Guo, J., Sun, Z., Zhang, L.,Weisel, R.D., Li, R.-K.Class II transactivator knockdown limitsmajor histocompatibility complex II expression, diminishes immune rejection, and improves survival of allogeneic bone marrow stem cells in the infarcted heart.
KW - Cell therapy
KW - Immune privilege
KW - Myocardial infarction
UR - http://www.scopus.com/inward/record.url?scp=84990842058&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84990842058&partnerID=8YFLogxK
U2 - 10.1096/fj.201600331R
DO - 10.1096/fj.201600331R
M3 - Article
C2 - 27221978
AN - SCOPUS:84990842058
SN - 0892-6638
VL - 30
SP - 3069
EP - 3082
JO - FASEB Journal
JF - FASEB Journal
IS - 9
ER -