TY - JOUR
T1 - Clerodendron glandulosum.Coleb extract ameliorates high fat diet/fatty acid induced lipotoxicity in experimental models of non-alcoholic steatohepatitis
AU - Jadeja, Ravirajsinh N.
AU - Thounaojam, Menaka C.
AU - Dandekar, Deven S.
AU - Devkar, Ranjitsinh V.
AU - Ramachandran, A. V.
N1 - Funding Information:
The authors (R.N.J and M.C.T) are grateful to University Grants Commission, New Delhi for providing Financial Assistance in the form of RFSMS scholarship. Thanks are also due to Dr. Prasanna S. Ghalsasi, Department of Chemistry, The M.S. University of Baroda, India, for kind help in spectroflorometer analysis and to Mr. Hemendra Vekaria, Department of Biochemistry, The M.S. University of Baroda, India, for providing DCFDA dye.
PY - 2010/12
Y1 - 2010/12
N2 - This study evaluates the protective role of Clerodendron glandulosum.Coleb (CG) aqueous extract against high fat diet/fatty acid induced lipotoxicity in experimental models of non-alcoholic steatohepatitis (NASH). Supplementation of NASH mice with CG extract (1% and 3% in high fat diet for 16. weeks) prevented high fat diet induced elevation in liver enzymes, plasma and hepatic lipids, mitochondrial oxidative stress and compromised enzymatic and non-enzymatic antioxidant status and histopathological damage to hepatocytes. Furthermore, results from in vitro study indicates, addition of CG extract (20-200 μg/ml for 24. h) to HepG2 cells minimizes oleic acid induced lipid accumulation, higher lipid peroxidation, cytotoxicity and reduced cell viability. These in vivo and in vitro studies suggest that CG extract has the potential of preventing high fat/fatty acid induced NASH.
AB - This study evaluates the protective role of Clerodendron glandulosum.Coleb (CG) aqueous extract against high fat diet/fatty acid induced lipotoxicity in experimental models of non-alcoholic steatohepatitis (NASH). Supplementation of NASH mice with CG extract (1% and 3% in high fat diet for 16. weeks) prevented high fat diet induced elevation in liver enzymes, plasma and hepatic lipids, mitochondrial oxidative stress and compromised enzymatic and non-enzymatic antioxidant status and histopathological damage to hepatocytes. Furthermore, results from in vitro study indicates, addition of CG extract (20-200 μg/ml for 24. h) to HepG2 cells minimizes oleic acid induced lipid accumulation, higher lipid peroxidation, cytotoxicity and reduced cell viability. These in vivo and in vitro studies suggest that CG extract has the potential of preventing high fat/fatty acid induced NASH.
KW - Clerodendron glandulosum .Coleb
KW - HepG2 cells
KW - Non-alcoholic steatohepatitis
KW - Oleic acid
KW - Oxidative stress
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UR - http://www.scopus.com/inward/citedby.url?scp=78049451156&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2010.09.015
DO - 10.1016/j.fct.2010.09.015
M3 - Article
C2 - 20849909
AN - SCOPUS:78049451156
SN - 0278-6915
VL - 48
SP - 3424
EP - 3431
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
IS - 12
ER -