TY - JOUR
T1 - Clinical characterization of in vivo inflammatory bowel disease with Raman spectroscopy
AU - Pence, Isaac J.
AU - Beaulieu, Dawn B.
AU - Horst, Sara N.
AU - Bi, Xiaohong
AU - Herline, Alan J.
AU - Schwartz, David A.
AU - Mahadevan-Jansen, Anita
N1 - Funding Information:
We would like to acknowledge funding support from American Society for Lasers in Medicine and Surgery student research grant and National Institutes of Health NRSA F31 predoctoral fellowship.
Publisher Copyright:
© 2017 Optical Society of America.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn’s disease (CD), affects over 1 million Americans and 2 million Europeans, and the incidence is increasing worldwide. While these diseases require unique medical care, the differentiation between UC and CD lacks a gold standard, and therefore relies on long term follow up, success or failure of existing treatment, and recurrence of the disease. Here, we present colonoscopy-coupled fiber optic probe-based Raman spectroscopy as a minimally-invasive diagnostic tool for IBD of the colon (UC and Crohn’s colitis). This pilot in vivo study of subjects with existing IBD diagnoses of UC (n = 8), CD (n = 15), and normal control (n = 8) aimed to characterize spectral signatures of UC and CD. Samples were correlated with tissue pathology markers and endoscopic evaluation. The collected spectra were processed and analyzed using multivariate statistical techniques to identify spectral markers and discriminate IBD and disease classes. Confounding factors including the presence of active inflammation and the particular colon segment measured were investigated and integrated into the devised prediction algorithm, reaching 90% sensitivity and 75% specificity to CD from this in vivo data set. These results represent significant progress towards improved real-time classification for accurate and automated in vivo detection and discrimination of IBD during colonoscopy procedures.
AB - Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn’s disease (CD), affects over 1 million Americans and 2 million Europeans, and the incidence is increasing worldwide. While these diseases require unique medical care, the differentiation between UC and CD lacks a gold standard, and therefore relies on long term follow up, success or failure of existing treatment, and recurrence of the disease. Here, we present colonoscopy-coupled fiber optic probe-based Raman spectroscopy as a minimally-invasive diagnostic tool for IBD of the colon (UC and Crohn’s colitis). This pilot in vivo study of subjects with existing IBD diagnoses of UC (n = 8), CD (n = 15), and normal control (n = 8) aimed to characterize spectral signatures of UC and CD. Samples were correlated with tissue pathology markers and endoscopic evaluation. The collected spectra were processed and analyzed using multivariate statistical techniques to identify spectral markers and discriminate IBD and disease classes. Confounding factors including the presence of active inflammation and the particular colon segment measured were investigated and integrated into the devised prediction algorithm, reaching 90% sensitivity and 75% specificity to CD from this in vivo data set. These results represent significant progress towards improved real-time classification for accurate and automated in vivo detection and discrimination of IBD during colonoscopy procedures.
KW - Medical optics instrumentation
KW - Raman spectroscopy
KW - Spectroscopy, tissue diagnostics
KW - Tissue characterization
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U2 - 10.1364/BOE.8.000524
DO - 10.1364/BOE.8.000524
M3 - Article
AN - SCOPUS:85011564541
SN - 2156-7085
VL - 8
SP - 524
EP - 535
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 2
M1 - #276220
ER -