Cloning of a novel kinase (SIK) of the SNF1/AMPK family from high salt diet-treated rat adrenal

Zhi Nong Wang; Hiroshi Takemori; Sunil Krishna Halder; Yasuki Nonaka; Mitsuhiro Okamoto

doi:10.1016/S0014-5793(99)00708-5

Cloning of a novel kinase (SIK) of the SNF1/AMPK family from high salt diet-treated rat adrenal

Zhi Nong Wang, Hiroshi Takemori, Sunil Krishna Halder, Yasuki Nonaka, Mitsuhiro Okamoto

Translational Research

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127 Scopus citations

Abstract

PCR-coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet-treated rat. A novel cDNA done, termed salt-inducible kinase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/threonine kinases in the SNF1/AMPK family was isolated. An in vitro kinase assay demonstrated that SIK protein had autophosphorylation activity. Northern blot revealed that SIK mRNA levels were markedly augmented by ACTH treatment both in rat adrenal glands and in Y1 cells. SIK may play an important role in the regulation of adrenocortical functions in response to high plasma salt and ACTH stimulation.

Original language	English (US)
Pages (from-to)	135-139
Number of pages	5
Journal	FEBS Letters
Volume	453
Issue number	1-2
DOIs	https://doi.org/10.1016/S0014-5793(99)00708-5
State	Published - Jun 18 1999

Keywords

Adrenocortical function
Adrenocorticotropic hormone
Protein serine/threonine kinase
SNF1/AMPK
Salt inducible kinase
Y1 mouse adrenocortical tumor cell

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(99)00708-5

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title = "Cloning of a novel kinase (SIK) of the SNF1/AMPK family from high salt diet-treated rat adrenal",

abstract = "PCR-coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet-treated rat. A novel cDNA done, termed salt-inducible kinase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/threonine kinases in the SNF1/AMPK family was isolated. An in vitro kinase assay demonstrated that SIK protein had autophosphorylation activity. Northern blot revealed that SIK mRNA levels were markedly augmented by ACTH treatment both in rat adrenal glands and in Y1 cells. SIK may play an important role in the regulation of adrenocortical functions in response to high plasma salt and ACTH stimulation.",

keywords = "Adrenocortical function, Adrenocorticotropic hormone, Protein serine/threonine kinase, SNF1/AMPK, Salt inducible kinase, Y1 mouse adrenocortical tumor cell",

author = "Wang, {Zhi Nong} and Hiroshi Takemori and Halder, {Sunil Krishna} and Yasuki Nonaka and Mitsuhiro Okamoto",

note = "Funding Information: A part of this study was supported by a research Grant-in-Aid from the Ministry of Education, Science, Sports and Culture of Japan.",

year = "1999",

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T1 - Cloning of a novel kinase (SIK) of the SNF1/AMPK family from high salt diet-treated rat adrenal

AU - Wang, Zhi Nong

AU - Takemori, Hiroshi

AU - Halder, Sunil Krishna

AU - Nonaka, Yasuki

AU - Okamoto, Mitsuhiro

N1 - Funding Information: A part of this study was supported by a research Grant-in-Aid from the Ministry of Education, Science, Sports and Culture of Japan.

PY - 1999/6/18

Y1 - 1999/6/18

N2 - PCR-coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet-treated rat. A novel cDNA done, termed salt-inducible kinase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/threonine kinases in the SNF1/AMPK family was isolated. An in vitro kinase assay demonstrated that SIK protein had autophosphorylation activity. Northern blot revealed that SIK mRNA levels were markedly augmented by ACTH treatment both in rat adrenal glands and in Y1 cells. SIK may play an important role in the regulation of adrenocortical functions in response to high plasma salt and ACTH stimulation.

AB - PCR-coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet-treated rat. A novel cDNA done, termed salt-inducible kinase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/threonine kinases in the SNF1/AMPK family was isolated. An in vitro kinase assay demonstrated that SIK protein had autophosphorylation activity. Northern blot revealed that SIK mRNA levels were markedly augmented by ACTH treatment both in rat adrenal glands and in Y1 cells. SIK may play an important role in the regulation of adrenocortical functions in response to high plasma salt and ACTH stimulation.

KW - Adrenocortical function

KW - Adrenocorticotropic hormone

KW - Protein serine/threonine kinase

KW - SNF1/AMPK

KW - Salt inducible kinase

KW - Y1 mouse adrenocortical tumor cell

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ER -

Cloning of a novel kinase (SIK) of the SNF1/AMPK family from high salt diet-treated rat adrenal

Abstract

Keywords

ASJC Scopus subject areas

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