Collagen-phosphorylcholine interpenetrating network hydrogels as corneal substitutes

Wenguang Liu; Chao Deng; Christopher R. McLaughlin; Per Fagerholm; Neil S. Lagali; Belinda Heyne; Juan C. Scaiano; Mitchell A. Watsky; Yasuhiro Kato; Rejean Munger; Naoshi Shinozaki; Fengfu Li; May Griffith

doi:10.1016/j.biomaterials.2008.11.022

Collagen-phosphorylcholine interpenetrating network hydrogels as corneal substitutes

Wenguang Liu, Chao Deng, Christopher R. McLaughlin, Per Fagerholm, Neil S. Lagali, Belinda Heyne, Juan C. Scaiano, Mitchell A. Watsky, Yasuhiro Kato, Rejean Munger, Naoshi Shinozaki, Fengfu Li, May Griffith

Research output: Contribution to journal › Article › peer-review

167 Scopus citations

Abstract

A biointeractive collagen-phospholipid corneal substitute was fabricated from interpenetrating polymeric networks comprising 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide and N-hydroxysuccinimide crosslinked porcine atelocollagen, and poly(ethylene glycol) diacrylate crosslinked 2-methacryloyloxyethyl phosphorylcholine (MPC). The resulting hydrogels showed an overall increase in mechanical strength beyond that of either original component and enhanced stability against enzymatic digestion (by collagenase) or UV degradation. More strikingly, these hydrogels retained the full biointeractive, cell friendly properties of collagen in promoting corneal cell and nerve in-growth and regeneration (despite MPC's known anti-adhesive properties). Measurements of refractive indices, white light transmission and backscatter showed the optical properties of collagen-MPC are comparable or superior to those of the human cornea. In addition, the glucose and albumin permeability were comparable to those of human corneas. Twelve-month post-implantation results of collagen-MPC hydrogels into mini-pigs showed regeneration of corneal tissue (epithelium, stroma) as well as the tear film and sensory nerves. We also show that porcine collagen can be substituted with recombinant human collagen, resulting in a fully-synthetic implant that is free from the potential risks of disease transmission (e.g. prions) present in animal source materials.

Original language	English (US)
Pages (from-to)	1551-1559
Number of pages	9
Journal	Biomaterials
Volume	30
Issue number	8
DOIs	https://doi.org/10.1016/j.biomaterials.2008.11.022
State	Published - Mar 2009
Externally published	Yes

Keywords

Biomedical regeneration
Cornea substitute
Phospholipid
Recombinant collagen
Tissue engineering

ASJC Scopus subject areas

Biophysics
Bioengineering
Ceramics and Composites
Biomaterials
Mechanics of Materials

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biomaterials.2008.11.022

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@article{ded01cc26f9445238c8e56fe8b18a59d,

title = "Collagen-phosphorylcholine interpenetrating network hydrogels as corneal substitutes",

abstract = "A biointeractive collagen-phospholipid corneal substitute was fabricated from interpenetrating polymeric networks comprising 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide and N-hydroxysuccinimide crosslinked porcine atelocollagen, and poly(ethylene glycol) diacrylate crosslinked 2-methacryloyloxyethyl phosphorylcholine (MPC). The resulting hydrogels showed an overall increase in mechanical strength beyond that of either original component and enhanced stability against enzymatic digestion (by collagenase) or UV degradation. More strikingly, these hydrogels retained the full biointeractive, cell friendly properties of collagen in promoting corneal cell and nerve in-growth and regeneration (despite MPC's known anti-adhesive properties). Measurements of refractive indices, white light transmission and backscatter showed the optical properties of collagen-MPC are comparable or superior to those of the human cornea. In addition, the glucose and albumin permeability were comparable to those of human corneas. Twelve-month post-implantation results of collagen-MPC hydrogels into mini-pigs showed regeneration of corneal tissue (epithelium, stroma) as well as the tear film and sensory nerves. We also show that porcine collagen can be substituted with recombinant human collagen, resulting in a fully-synthetic implant that is free from the potential risks of disease transmission (e.g. prions) present in animal source materials.",

keywords = "Biomedical regeneration, Cornea substitute, Phospholipid, Recombinant collagen, Tissue engineering",

author = "Wenguang Liu and Chao Deng and McLaughlin, {Christopher R.} and Per Fagerholm and Lagali, {Neil S.} and Belinda Heyne and Scaiano, {Juan C.} and Watsky, {Mitchell A.} and Yasuhiro Kato and Rejean Munger and Naoshi Shinozaki and Fengfu Li and May Griffith",

note = "Funding Information: We thank Mr. Minoru Fukuda at Kyorin University for excellent TEM assistance and Subhadra Dravida for technical assistance with immunohistochemistry. Funding support from NSERC and CIHR Canada CHRP Grant No. 337436 to M. Griffith and J.C. Scaiano; NSERC studentship to C.R. McLaughlin.",

year = "2009",

month = mar,

doi = "10.1016/j.biomaterials.2008.11.022",

language = "English (US)",

volume = "30",

pages = "1551--1559",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier BV",

number = "8",

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TY - JOUR

T1 - Collagen-phosphorylcholine interpenetrating network hydrogels as corneal substitutes

AU - Liu, Wenguang

AU - Deng, Chao

AU - McLaughlin, Christopher R.

AU - Fagerholm, Per

AU - Lagali, Neil S.

AU - Heyne, Belinda

AU - Scaiano, Juan C.

AU - Watsky, Mitchell A.

AU - Kato, Yasuhiro

AU - Munger, Rejean

AU - Shinozaki, Naoshi

AU - Li, Fengfu

AU - Griffith, May

N1 - Funding Information: We thank Mr. Minoru Fukuda at Kyorin University for excellent TEM assistance and Subhadra Dravida for technical assistance with immunohistochemistry. Funding support from NSERC and CIHR Canada CHRP Grant No. 337436 to M. Griffith and J.C. Scaiano; NSERC studentship to C.R. McLaughlin.

PY - 2009/3

Y1 - 2009/3

N2 - A biointeractive collagen-phospholipid corneal substitute was fabricated from interpenetrating polymeric networks comprising 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide and N-hydroxysuccinimide crosslinked porcine atelocollagen, and poly(ethylene glycol) diacrylate crosslinked 2-methacryloyloxyethyl phosphorylcholine (MPC). The resulting hydrogels showed an overall increase in mechanical strength beyond that of either original component and enhanced stability against enzymatic digestion (by collagenase) or UV degradation. More strikingly, these hydrogels retained the full biointeractive, cell friendly properties of collagen in promoting corneal cell and nerve in-growth and regeneration (despite MPC's known anti-adhesive properties). Measurements of refractive indices, white light transmission and backscatter showed the optical properties of collagen-MPC are comparable or superior to those of the human cornea. In addition, the glucose and albumin permeability were comparable to those of human corneas. Twelve-month post-implantation results of collagen-MPC hydrogels into mini-pigs showed regeneration of corneal tissue (epithelium, stroma) as well as the tear film and sensory nerves. We also show that porcine collagen can be substituted with recombinant human collagen, resulting in a fully-synthetic implant that is free from the potential risks of disease transmission (e.g. prions) present in animal source materials.

AB - A biointeractive collagen-phospholipid corneal substitute was fabricated from interpenetrating polymeric networks comprising 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide and N-hydroxysuccinimide crosslinked porcine atelocollagen, and poly(ethylene glycol) diacrylate crosslinked 2-methacryloyloxyethyl phosphorylcholine (MPC). The resulting hydrogels showed an overall increase in mechanical strength beyond that of either original component and enhanced stability against enzymatic digestion (by collagenase) or UV degradation. More strikingly, these hydrogels retained the full biointeractive, cell friendly properties of collagen in promoting corneal cell and nerve in-growth and regeneration (despite MPC's known anti-adhesive properties). Measurements of refractive indices, white light transmission and backscatter showed the optical properties of collagen-MPC are comparable or superior to those of the human cornea. In addition, the glucose and albumin permeability were comparable to those of human corneas. Twelve-month post-implantation results of collagen-MPC hydrogels into mini-pigs showed regeneration of corneal tissue (epithelium, stroma) as well as the tear film and sensory nerves. We also show that porcine collagen can be substituted with recombinant human collagen, resulting in a fully-synthetic implant that is free from the potential risks of disease transmission (e.g. prions) present in animal source materials.

KW - Biomedical regeneration

KW - Cornea substitute

KW - Phospholipid

KW - Recombinant collagen

KW - Tissue engineering

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UR - http://www.scopus.com/inward/citedby.url?scp=58249089265&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2008.11.022

DO - 10.1016/j.biomaterials.2008.11.022

M3 - Article

C2 - 19097643

AN - SCOPUS:58249089265

SN - 0142-9612

VL - 30

SP - 1551

EP - 1559

JO - Biomaterials

JF - Biomaterials

IS - 8

ER -

Collagen-phosphorylcholine interpenetrating network hydrogels as corneal substitutes

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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