TY - JOUR
T1 - Comparative STAT3-regulated gene expression profile in renal cell carcinoma subtypes
AU - Robinson, Rebekah L.
AU - Sharma, Ashok Kumar
AU - Bai, Shan
AU - Heneidi, Saleh
AU - Lee, Tae Jin
AU - Kodeboyina, Sai Karthik
AU - Patel, Nikhil
AU - Sharma, Shruti
N1 - Funding Information:
This research was supported by Institutional Start-Up Package to SS from Medical College of Georgia at Augusta University, Augusta, GA, USA.
Publisher Copyright:
Copyright © 2019 Robinson, Sharma, Bai, Heneidi, Lee, Kodeboyina, Patel and Sharma.
PY - 2019
Y1 - 2019
N2 - Renal cell carcinomas (RCC) are heterogeneous and can be further classified into three major subtypes including clear cell, papillary and chromophobe. Signal transducer and activator of transcription 3 (STAT3) is commonly hyperactive in many cancers and is associated with cancer cell proliferation, invasion, migration, and angiogenesis. In renal cell carcinoma, increased STAT3 activation is associated with increased metastasis and worse survival outcomes, but clinical trials targeting the STAT3 signaling pathway have shown varying levels of success in different RCC subtypes. Using RNA-seq data from The Cancer Genome Atlas (TCGA), we compared expression of 32 STAT3 regulated genes in 3 RCC subtypes. Our results indicate that STAT3 activation plays the most significant role in clear cell RCC relative to the other subtypes, as half of the evaluated genes were upregulated in this subtype. MMP9, BIRC5, and BCL2 were upregulated and FOS was downregulated in all three subtypes. Several genes including VEGFA, VIM, MYC, ITGB4, ICAM1, MMP1, CCND1, STMN1, TWIST1, and PIM2 had variable expression in RCC subtypes and are potential therapeutic targets for personalized medicine.
AB - Renal cell carcinomas (RCC) are heterogeneous and can be further classified into three major subtypes including clear cell, papillary and chromophobe. Signal transducer and activator of transcription 3 (STAT3) is commonly hyperactive in many cancers and is associated with cancer cell proliferation, invasion, migration, and angiogenesis. In renal cell carcinoma, increased STAT3 activation is associated with increased metastasis and worse survival outcomes, but clinical trials targeting the STAT3 signaling pathway have shown varying levels of success in different RCC subtypes. Using RNA-seq data from The Cancer Genome Atlas (TCGA), we compared expression of 32 STAT3 regulated genes in 3 RCC subtypes. Our results indicate that STAT3 activation plays the most significant role in clear cell RCC relative to the other subtypes, as half of the evaluated genes were upregulated in this subtype. MMP9, BIRC5, and BCL2 were upregulated and FOS was downregulated in all three subtypes. Several genes including VEGFA, VIM, MYC, ITGB4, ICAM1, MMP1, CCND1, STMN1, TWIST1, and PIM2 had variable expression in RCC subtypes and are potential therapeutic targets for personalized medicine.
KW - Gene expression
KW - RNA-seq
KW - Renal cell carcinoma
KW - STAT3
KW - TCGA
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U2 - 10.3389/fonc.2019.00072
DO - 10.3389/fonc.2019.00072
M3 - Article
AN - SCOPUS:85063272778
SN - 2234-943X
VL - 9
JO - Frontiers in Oncology
JF - Frontiers in Oncology
IS - FEB
M1 - 72
ER -