Comparison of ancient and modern Clonorchis sinensis based on ITS1 and ITS2 sequences

Wen Qi Liu, Juan Liu, Jun Hua Zhang, Xiao Chun Long, Jia Hui Lei, Yong Long Li

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


In 1975, an ancient corpse buried in 167 BC was found at Jiangling County, Hubei Province of China. The eggs of Clonorchis sinensis found in the gall bladder of the corpse were preserved well. In the present paper, we extracted the genomic DNA from the ancient eggs and modern eggs, respectively, and the internal transcribed spacer 1 and 2 (ITS1 and ITS2) at ribosomal RNA genes were studied. The results show that ITS2 sequences from the ancient sample were identical with those from modern samples, but in ITS1 differences in 15 nucleotide positions were found between the ancient and modern samples. The results demonstrated that it is possible to extract and sequence DNA from ancient parasite eggs. The ITS1 sequence obtained differed from all modern ones available to date. This might indicate sequence divergence through time, or might reflect a sequence polymorphism that may eventually be found also in modern samples.

Original languageEnglish (US)
Pages (from-to)91-94
Number of pages4
JournalActa Tropica
Issue number2
StatePublished - Feb 2007
Externally publishedYes


  • Clonorchis sinensis
  • ITS1 and ITS2
  • Sequence divergence

ASJC Scopus subject areas

  • Parasitology
  • veterinary (miscalleneous)
  • Insect Science
  • Infectious Diseases


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