Comparison of ancient and modern Clonorchis sinensis based on ITS1 and ITS2 sequences

Wen Qi Liu; Juan Liu; Jun Hua Zhang; Xiao Chun Long; Jia Hui Lei; Yong Long Li

doi:10.1016/j.actatropica.2006.08.010

Comparison of ancient and modern Clonorchis sinensis based on ITS1 and ITS2 sequences

Wen Qi Liu, Juan Liu, Jun Hua Zhang, Xiao Chun Long, Jia Hui Lei, Yong Long Li

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

In 1975, an ancient corpse buried in 167 BC was found at Jiangling County, Hubei Province of China. The eggs of Clonorchis sinensis found in the gall bladder of the corpse were preserved well. In the present paper, we extracted the genomic DNA from the ancient eggs and modern eggs, respectively, and the internal transcribed spacer 1 and 2 (ITS1 and ITS2) at ribosomal RNA genes were studied. The results show that ITS2 sequences from the ancient sample were identical with those from modern samples, but in ITS1 differences in 15 nucleotide positions were found between the ancient and modern samples. The results demonstrated that it is possible to extract and sequence DNA from ancient parasite eggs. The ITS1 sequence obtained differed from all modern ones available to date. This might indicate sequence divergence through time, or might reflect a sequence polymorphism that may eventually be found also in modern samples.

Original language	English (US)
Pages (from-to)	91-94
Number of pages	4
Journal	Acta Tropica
Volume	101
Issue number	2
DOIs	https://doi.org/10.1016/j.actatropica.2006.08.010
State	Published - Feb 2007
Externally published	Yes

Keywords

Clonorchis sinensis
ITS1 and ITS2
Sequence divergence

ASJC Scopus subject areas

Parasitology
veterinary (miscalleneous)
Insect Science
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.actatropica.2006.08.010

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title = "Comparison of ancient and modern Clonorchis sinensis based on ITS1 and ITS2 sequences",

abstract = "In 1975, an ancient corpse buried in 167 BC was found at Jiangling County, Hubei Province of China. The eggs of Clonorchis sinensis found in the gall bladder of the corpse were preserved well. In the present paper, we extracted the genomic DNA from the ancient eggs and modern eggs, respectively, and the internal transcribed spacer 1 and 2 (ITS1 and ITS2) at ribosomal RNA genes were studied. The results show that ITS2 sequences from the ancient sample were identical with those from modern samples, but in ITS1 differences in 15 nucleotide positions were found between the ancient and modern samples. The results demonstrated that it is possible to extract and sequence DNA from ancient parasite eggs. The ITS1 sequence obtained differed from all modern ones available to date. This might indicate sequence divergence through time, or might reflect a sequence polymorphism that may eventually be found also in modern samples.",

keywords = "Clonorchis sinensis, ITS1 and ITS2, Sequence divergence",

author = "Liu, {Wen Qi} and Juan Liu and Zhang, {Jun Hua} and Long, {Xiao Chun} and Lei, {Jia Hui} and Li, {Yong Long}",

note = "Funding Information: We thank Prof. Dr. Malcolm W. Kennedy in University of Glasgow and Prof. Dr. Andreas Ruppel in University of Heidelberg for their critical reading of this manuscript. This work was supported by National Natural Foundation of China (project grant number 30370209) and State Key Laboratory of Paleobiology and Stratigraphy, CAS (project grant number 023112).",

year = "2007",

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doi = "10.1016/j.actatropica.2006.08.010",

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AU - Liu, Wen Qi

AU - Liu, Juan

AU - Zhang, Jun Hua

AU - Long, Xiao Chun

AU - Lei, Jia Hui

AU - Li, Yong Long

N1 - Funding Information: We thank Prof. Dr. Malcolm W. Kennedy in University of Glasgow and Prof. Dr. Andreas Ruppel in University of Heidelberg for their critical reading of this manuscript. This work was supported by National Natural Foundation of China (project grant number 30370209) and State Key Laboratory of Paleobiology and Stratigraphy, CAS (project grant number 023112).

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Y1 - 2007/2

N2 - In 1975, an ancient corpse buried in 167 BC was found at Jiangling County, Hubei Province of China. The eggs of Clonorchis sinensis found in the gall bladder of the corpse were preserved well. In the present paper, we extracted the genomic DNA from the ancient eggs and modern eggs, respectively, and the internal transcribed spacer 1 and 2 (ITS1 and ITS2) at ribosomal RNA genes were studied. The results show that ITS2 sequences from the ancient sample were identical with those from modern samples, but in ITS1 differences in 15 nucleotide positions were found between the ancient and modern samples. The results demonstrated that it is possible to extract and sequence DNA from ancient parasite eggs. The ITS1 sequence obtained differed from all modern ones available to date. This might indicate sequence divergence through time, or might reflect a sequence polymorphism that may eventually be found also in modern samples.

AB - In 1975, an ancient corpse buried in 167 BC was found at Jiangling County, Hubei Province of China. The eggs of Clonorchis sinensis found in the gall bladder of the corpse were preserved well. In the present paper, we extracted the genomic DNA from the ancient eggs and modern eggs, respectively, and the internal transcribed spacer 1 and 2 (ITS1 and ITS2) at ribosomal RNA genes were studied. The results show that ITS2 sequences from the ancient sample were identical with those from modern samples, but in ITS1 differences in 15 nucleotide positions were found between the ancient and modern samples. The results demonstrated that it is possible to extract and sequence DNA from ancient parasite eggs. The ITS1 sequence obtained differed from all modern ones available to date. This might indicate sequence divergence through time, or might reflect a sequence polymorphism that may eventually be found also in modern samples.

KW - Clonorchis sinensis

KW - ITS1 and ITS2

KW - Sequence divergence

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Comparison of ancient and modern Clonorchis sinensis based on ITS1 and ITS2 sequences

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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