TY - JOUR
T1 - Comparison of the Accessory Activity of Murine Peritoneal Cavity Macrophage Derived Dendritic Cells and Peritoneal Cavity Macrophages in a Mixed Lymphocyte Reaction
AU - Makala, Levi H
AU - Nishikawa, Yoshifumi
AU - Kamada, Takenori
AU - Suzuki, Hiroshi
AU - Xuan, Xuenan
AU - Igarashi, Ikuo
AU - Nagasawa, Hideyuki
PY - 2001/12
Y1 - 2001/12
N2 - A detailed comparison of the accessory cell activities was carried out among murine peritoneal cavity macrophages (PEC-MΦ). peritoneal cavity macrophages stimulated with granulocyte-macrophage colony stimulating factor (GM-CSF) plus interleukin 4 (IL-4), the most popular cytokine combination widely used to generate dendritic cells (DC) and peritoneal cavity macrophage-derived DC (PEC-DC) using a two-way mixed lymphocyte reaction (MLR). All the cell types used efficiently induced statistically significant naïve T cell proliferation at all culture time points and responder:stimulator ratios used. However, marked differences were noted in the magnitude of the proliferative responses. These variations may be attributed to the intensity of expression of MHC class II glycoproteins, as well as the actual numbers of MHC class II+ cells.
AB - A detailed comparison of the accessory cell activities was carried out among murine peritoneal cavity macrophages (PEC-MΦ). peritoneal cavity macrophages stimulated with granulocyte-macrophage colony stimulating factor (GM-CSF) plus interleukin 4 (IL-4), the most popular cytokine combination widely used to generate dendritic cells (DC) and peritoneal cavity macrophage-derived DC (PEC-DC) using a two-way mixed lymphocyte reaction (MLR). All the cell types used efficiently induced statistically significant naïve T cell proliferation at all culture time points and responder:stimulator ratios used. However, marked differences were noted in the magnitude of the proliferative responses. These variations may be attributed to the intensity of expression of MHC class II glycoproteins, as well as the actual numbers of MHC class II+ cells.
KW - Mixed lymphocyte reaction
KW - Peritoneal cavity macrophage
KW - Peritoneal cavity macrophage-derived dendritic cell
UR - http://www.scopus.com/inward/record.url?scp=0035755741&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035755741&partnerID=8YFLogxK
U2 - 10.1292/jvms.63.1271
DO - 10.1292/jvms.63.1271
M3 - Article
C2 - 11789603
AN - SCOPUS:0035755741
SN - 0916-7250
VL - 63
SP - 1271
EP - 1277
JO - Journal of Veterinary Medical Science
JF - Journal of Veterinary Medical Science
IS - 12
ER -