TY - JOUR
T1 - Comparison of three methods of critical evaluation of microleakage along restorative interfaces
AU - Gwinnett, John A.
AU - Tay, Franklin R.
AU - Pang, K. M.
AU - Wei, Stephen H.Y.
N1 - Funding Information:
State University of New York at Stony Brook, Stony Brook, N. Y, and The University of Hong Kong, Hong Kong This in vitro study compared microleakage along the dentin-restorative interface by use of a conventional single-surface and two stereoscopical protocols. The extent of microleakage in class V cylindric cavities that were restored with Scotchbond 2/P-50 was assessed after thermocycling and silver staining were performed. Specimens were randomized into three groups of 10 teeth for the three different protocols to be investigated. The categories were group 1, a single longitudinal section through the midline of the restoration, group 2, a multiple-surface protocol based on computer reconstruction of digitized images from multiple serial abrasions, and group 3, a clearing protocol based on immersion of demineralized specimens in methyl salicylate. The severity of microleakage for all groups was recorded on an ordinal scale and based on linear leakage length. The median leakage score for groups 1 through 3 was 2, 4, and 4, respectively. When comparisons were performed with nonparametric statistical analysis, both the clearing and the multiple-surface protocols recorded significantly more severe leakage than those detected from a conventional, single longitudinal, midline sectioning technique, (x 2 = 8.595, p < 0.02). Furthermore mi-croleakage patterns generated from the two stereoscopical protocols revealed extensive microleakage that advanced unilaterally or bilaterally through the dentin-restorative interface along the embrasure regions into the axial wall of the restorations. No significant difference was noted between the two stereoscopical protocols (Q = 0.260, p > 0.05), which suggested that the clearing protocol, which is less labor-intensive than the multiple-surface protocol, may be recommended for future comparative microleakage studies that involve the dentin-restorative interface. (J PROSTHET DENT 1995;74:575-85.) The integrity and durability of the marginal seal have always been prime concerns in the investigation of the performance of dental restorative materials. The clinically undetectable passage of bacteria, fluids, molecules, or ions between the cavity wall and the applied restorative material has been defined as microleakage. 1 Clinically, microleakage may be identified as a dynamic phenomenon that results in two consequential manifestations. A com- Supported by the Committee on Research and Conference Grants (CRCG), the University of Hong Kong. aProfessor, Department of Oral Biology and Pathology, School of Dental Medicine. bClinical Lecturer, Department of Children's Dentistry and Orth-odontics, Prince Philip Dental Hospital, The University of Hong Kong. ~Chief Technician, Oral Biology Unit, Faculty of Dentistry, The University of Hong Kong. dDean and Professor, Department of Children's Dentistry and Orthodontics, Prince Philip Dental Hospital, The University of Hong Kong. Copyright 9 1995 by The Editorial Council of THE JOURNAL OF PROSTHETIC DENTISTRY. 0022-3913/95/$5.00 + 0. 1011/67360 promised marginal seal causes hydrodynamic fluid movement through a degrading smear layer into the patent dentinal tubules underneath 2 to result in hypersensitivity to thermal and osmotic stimuli and is referred to as the sensory component of microleakage. 3 Penetration of bacteria and their products through such potential gaps along the axiopulpal floor accounts for the pathologic component ofmicroleakage that results in recurrent caries and subsequent pulpal pathoses. 4 A correlation between material biocompatibility and pulp health in the absence of mi-croleakage and bacterial infection was reported. 5, 6 Different methods that were used to study microleakage around dental restorations have been reviewed, 1, 7-12 and among these the use of silver nitrate and various dye penetration remained the most popular for in vitro microleak-age evaluations. In these studies the magnitude of leakage was most commonly assessed ai~r hemisection of the specimens was performed longitudinally through the mid-line of the restoration. The severity of microleakage was delineated as an ordinal score 13 or a linear leakage length. 14 Image analysis has been used to describe the leakage area along the interface, 15 but regardless of the level of sophistication of the measurements subsequently used, the major problem associated with interpretation of
PY - 1995/12
Y1 - 1995/12
N2 - This in vitro study compared microleakage along the dentin-restorative interface by use of a conventional single-surface and two stereoscopical protocols. The extent of microleakage in class V cylindric cavities that were restored with Scotchbond 2/P-50 was assessed after thermocycling and silver staining were performed. Specimens were randomized into three groups of 10 teeth for the three different protocols to be investigated. The categories were group 1, a single longitudinal section through the midline of the restoration, group 2, a multiple-surface protocol based on computer reconstruction of digitized images from multiple serial abrasions, and group 3, a clearing protocol based on immersion of demineralized specimens in methyl salicylate. The severity of microleakage for all groups was recorded on an ordinal scale and based on linear leakage length. The median leakage score for groups 1 through 3 was 2, 4, and 4, respectively. When comparisons were performed with nonparametric statistical analysis, both the clearing and the multiple-surface protocols recorded significantly more severe leakage than those detected from a conventional, single longitudinal, midline sectioning technique, (x2=8.595, p<0.02). Furthermore microleakage patterns generated from the two stereoscopical protocols revealed extensive microleakage that advanced unilaterally or bilaterally through the dentin-restorative interface along the embrasure regions into the axial wall of the restorations. No significant difference was noted between the two stereoscopical protocols (Q=0.260, p>0.05), which suggested that the clearing protocol, which is less labor-intensive than the multiple-surface protocol, may be recommended for future comparative microleakage studies that involve the dentin-restorative interface.
AB - This in vitro study compared microleakage along the dentin-restorative interface by use of a conventional single-surface and two stereoscopical protocols. The extent of microleakage in class V cylindric cavities that were restored with Scotchbond 2/P-50 was assessed after thermocycling and silver staining were performed. Specimens were randomized into three groups of 10 teeth for the three different protocols to be investigated. The categories were group 1, a single longitudinal section through the midline of the restoration, group 2, a multiple-surface protocol based on computer reconstruction of digitized images from multiple serial abrasions, and group 3, a clearing protocol based on immersion of demineralized specimens in methyl salicylate. The severity of microleakage for all groups was recorded on an ordinal scale and based on linear leakage length. The median leakage score for groups 1 through 3 was 2, 4, and 4, respectively. When comparisons were performed with nonparametric statistical analysis, both the clearing and the multiple-surface protocols recorded significantly more severe leakage than those detected from a conventional, single longitudinal, midline sectioning technique, (x2=8.595, p<0.02). Furthermore microleakage patterns generated from the two stereoscopical protocols revealed extensive microleakage that advanced unilaterally or bilaterally through the dentin-restorative interface along the embrasure regions into the axial wall of the restorations. No significant difference was noted between the two stereoscopical protocols (Q=0.260, p>0.05), which suggested that the clearing protocol, which is less labor-intensive than the multiple-surface protocol, may be recommended for future comparative microleakage studies that involve the dentin-restorative interface.
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U2 - 10.1016/S0022-3913(05)80308-7
DO - 10.1016/S0022-3913(05)80308-7
M3 - Article
C2 - 8778380
AN - SCOPUS:0029439363
SN - 0022-3913
VL - 74
SP - 575
EP - 585
JO - The Journal of Prosthetic Dentistry
JF - The Journal of Prosthetic Dentistry
IS - 6
ER -