TY - JOUR
T1 - Compensation for partial lipectomy in mice with genetic alterations of leptin and its receptor subtypes
AU - Harris, Ruth B.S.
AU - Hausman, Dorothy B.
AU - Bartness, Timothy J.
PY - 2002/11/1
Y1 - 2002/11/1
N2 - One hypothesis for the regulation of total body fat suggests that leptin is a lipostatic feedback signal that acts at brain sites involved in regulation of energy balance. The importance of leptin in recovery from partial surgical lipectomy was tested by performing bilateral epididymal lipectomy or sham surgery on wild-type and leptin-deficient ob/ob mice. Eight weeks later, nonexcised pads of lipectomized mice were increased but total carcass fat was lower than in sham-operated ob/ob mice. In experiment 2, ob/ob mice, wild-type mice, and two db/db mutants, C57BL/6J dbLepr/ dbLepr (BL/6J) mice possessing short-form and circulating leptin receptors and C57BL/6J db3J/db3J (BL/3J) mice expressing only circulating receptors, were lipectomized or sham operated. Sixteen weeks later, body mass and carcass lipid were not different between sham and lipectomized ob/ob mice, wild-type mice, or BL/6J db/db mice, whereas there was incomplete (decreased carcass fat) but suggestive recovery (increased retroperitoneal fat mass and cell number) in lipectomized BL/3J db/db mice. These data indicate that leptin is not required for the regulation of total body fat.
AB - One hypothesis for the regulation of total body fat suggests that leptin is a lipostatic feedback signal that acts at brain sites involved in regulation of energy balance. The importance of leptin in recovery from partial surgical lipectomy was tested by performing bilateral epididymal lipectomy or sham surgery on wild-type and leptin-deficient ob/ob mice. Eight weeks later, nonexcised pads of lipectomized mice were increased but total carcass fat was lower than in sham-operated ob/ob mice. In experiment 2, ob/ob mice, wild-type mice, and two db/db mutants, C57BL/6J dbLepr/ dbLepr (BL/6J) mice possessing short-form and circulating leptin receptors and C57BL/6J db3J/db3J (BL/3J) mice expressing only circulating receptors, were lipectomized or sham operated. Sixteen weeks later, body mass and carcass lipid were not different between sham and lipectomized ob/ob mice, wild-type mice, or BL/6J db/db mice, whereas there was incomplete (decreased carcass fat) but suggestive recovery (increased retroperitoneal fat mass and cell number) in lipectomized BL/3J db/db mice. These data indicate that leptin is not required for the regulation of total body fat.
KW - Cell size distribution
KW - Db/db mice
KW - Ob/ob mice
KW - Total body fat
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U2 - 10.1152/ajpregu.00339.2002
DO - 10.1152/ajpregu.00339.2002
M3 - Article
C2 - 12376403
AN - SCOPUS:0036838474
SN - 0363-6119
VL - 283
SP - R1094-R1103
JO - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
JF - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
IS - 5 52-5
ER -