Curcumin-loaded embryonic stem cell exosomes restored neurovascular unit following ischemia-reperfusion injury

Anuradha Kalani; Pankaj Chaturvedi; Pradip K. Kamat; Claudio Maldonado; Philip Bauer; Irving G. Joshua; Suresh C. Tyagi; Neetu Tyagi

doi:10.1016/j.biocel.2016.09.002

Curcumin-loaded embryonic stem cell exosomes restored neurovascular unit following ischemia-reperfusion injury

Anuradha Kalani, Pankaj Chaturvedi, Pradip K. Kamat, Claudio Maldonado, Philip Bauer, Irving G. Joshua, Suresh C. Tyagi, Neetu Tyagi

Research output: Contribution to journal › Article › peer-review

192 Scopus citations

Abstract

We tested whether the combined nano-formulation, prepared with curcumin (anti-inflammatory and neuroprotective molecule) and embryonic stem cell exosomes (MESC-exo^cur), restored neurovascular loss following an ischemia reperfusion (IR) injury in mice. IR-injury was created in 8–10 weeks old mice and divided into two groups. Out of two IR-injured groups, one group received intranasal administration of MESC-exo^cur for 7 days. Similarly, two sham groups were made and one group received MESC-exo^cur treatment. The study determined that MESC-exo^cur treatment reduced neurological score, infarct volume and edema following IR-injury. As compared to untreated IR group, MESC-exo^cur treated-IR group showed reduced inflammation and N-methyl-D-aspartate receptor expression. Treatment of MESC-exo^cur also reduced astrocytic GFAP expression and alleviated the expression of NeuN positive neurons in IR-injured mice. In addition, MESC-exo^cur treatment restored vascular endothelial tight (claudin-5 and occludin) and adherent (VE-cadherin) junction proteins in IR-injured mice as compared to untreated IR-injured mice. These results suggest that combining the potentials of embryonic stem cell exosomes and curcumin can help neurovascular restoration following ischemia-reperfusion injury in mice.

Original language	English (US)
Pages (from-to)	360-369
Number of pages	10
Journal	International Journal of Biochemistry and Cell Biology
Volume	79
DOIs	https://doi.org/10.1016/j.biocel.2016.09.002
State	Published - Oct 1 2016
Externally published	Yes

Keywords

Curcumin
Exosomes
Neurons
Stroke

ASJC Scopus subject areas

Biochemistry
Cell Biology

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10.1016/j.biocel.2016.09.002

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title = "Curcumin-loaded embryonic stem cell exosomes restored neurovascular unit following ischemia-reperfusion injury",

abstract = "We tested whether the combined nano-formulation, prepared with curcumin (anti-inflammatory and neuroprotective molecule) and embryonic stem cell exosomes (MESC-exocur), restored neurovascular loss following an ischemia reperfusion (IR) injury in mice. IR-injury was created in 8–10 weeks old mice and divided into two groups. Out of two IR-injured groups, one group received intranasal administration of MESC-exocur for 7 days. Similarly, two sham groups were made and one group received MESC-exocur treatment. The study determined that MESC-exocur treatment reduced neurological score, infarct volume and edema following IR-injury. As compared to untreated IR group, MESC-exocur treated-IR group showed reduced inflammation and N-methyl-D-aspartate receptor expression. Treatment of MESC-exocur also reduced astrocytic GFAP expression and alleviated the expression of NeuN positive neurons in IR-injured mice. In addition, MESC-exocur treatment restored vascular endothelial tight (claudin-5 and occludin) and adherent (VE-cadherin) junction proteins in IR-injured mice as compared to untreated IR-injured mice. These results suggest that combining the potentials of embryonic stem cell exosomes and curcumin can help neurovascular restoration following ischemia-reperfusion injury in mice.",

keywords = "Curcumin, Exosomes, Neurons, Stroke",

author = "Anuradha Kalani and Pankaj Chaturvedi and Kamat, {Pradip K.} and Claudio Maldonado and Philip Bauer and Joshua, {Irving G.} and Tyagi, {Suresh C.} and Neetu Tyagi",

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AU - Kalani, Anuradha

AU - Chaturvedi, Pankaj

AU - Kamat, Pradip K.

AU - Maldonado, Claudio

AU - Bauer, Philip

AU - Joshua, Irving G.

AU - Tyagi, Suresh C.

AU - Tyagi, Neetu

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AB - We tested whether the combined nano-formulation, prepared with curcumin (anti-inflammatory and neuroprotective molecule) and embryonic stem cell exosomes (MESC-exocur), restored neurovascular loss following an ischemia reperfusion (IR) injury in mice. IR-injury was created in 8–10 weeks old mice and divided into two groups. Out of two IR-injured groups, one group received intranasal administration of MESC-exocur for 7 days. Similarly, two sham groups were made and one group received MESC-exocur treatment. The study determined that MESC-exocur treatment reduced neurological score, infarct volume and edema following IR-injury. As compared to untreated IR group, MESC-exocur treated-IR group showed reduced inflammation and N-methyl-D-aspartate receptor expression. Treatment of MESC-exocur also reduced astrocytic GFAP expression and alleviated the expression of NeuN positive neurons in IR-injured mice. In addition, MESC-exocur treatment restored vascular endothelial tight (claudin-5 and occludin) and adherent (VE-cadherin) junction proteins in IR-injured mice as compared to untreated IR-injured mice. These results suggest that combining the potentials of embryonic stem cell exosomes and curcumin can help neurovascular restoration following ischemia-reperfusion injury in mice.

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KW - Neurons

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Curcumin-loaded embryonic stem cell exosomes restored neurovascular unit following ischemia-reperfusion injury

Abstract

Keywords

ASJC Scopus subject areas

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