TY - JOUR
T1 - CXCR7 protein is not expressed on human or mouse leukocytes
AU - Berahovich, Robert D.
AU - Zabel, Brian A.
AU - Penfold, Mark E.T.
AU - Lewén, Susanna
AU - Wang, Yu
AU - Miao, Zhenhua
AU - Gan, Lin
AU - Pereda, Jaime
AU - Dias, Jessica
AU - Slukvin, Igor I.
AU - McGrath, Kathleen E.
AU - Jaen, Juan C.
AU - Schall, Thomas J.
N1 - Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2010/11/1
Y1 - 2010/11/1
N2 - Since the discovery that CXCR7 binds to CXCL12/SDF-1α, the role of CXCR7 in CXCL12-mediated biological processes has been under intensive scrutiny. However, there is no consensus in the literature on the expression of CXCR7 protein by peripheral blood cells. In this study we analyzed human and mouse leukocytes and erythrocytes for CXCR7 protein expression, using a competitive CXCL12 binding assay as well as by flow cytometry and immunohistochemistry using multiple CXCR7 Abs. CXCR7-/- mice were used as negative controls. Together, these methods indicate that CXCR7 protein is not expressed by human peripheral blood T cells, B cells, NK cells, or monocytes, or by mouse peripheral blood leukocytes. CXCR7 protein is, however, expressed on mouse primitive erythroid cells, which supply oxygen to the embryo during early stages of development. These studies therefore suggest that, whereas CXCR7 protein is expressed by primitive RBCs during murine embryonic development, in adult mammals CXCR7 protein is not expressed by normal peripheral blood cells.
AB - Since the discovery that CXCR7 binds to CXCL12/SDF-1α, the role of CXCR7 in CXCL12-mediated biological processes has been under intensive scrutiny. However, there is no consensus in the literature on the expression of CXCR7 protein by peripheral blood cells. In this study we analyzed human and mouse leukocytes and erythrocytes for CXCR7 protein expression, using a competitive CXCL12 binding assay as well as by flow cytometry and immunohistochemistry using multiple CXCR7 Abs. CXCR7-/- mice were used as negative controls. Together, these methods indicate that CXCR7 protein is not expressed by human peripheral blood T cells, B cells, NK cells, or monocytes, or by mouse peripheral blood leukocytes. CXCR7 protein is, however, expressed on mouse primitive erythroid cells, which supply oxygen to the embryo during early stages of development. These studies therefore suggest that, whereas CXCR7 protein is expressed by primitive RBCs during murine embryonic development, in adult mammals CXCR7 protein is not expressed by normal peripheral blood cells.
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U2 - 10.4049/jimmunol.1001660
DO - 10.4049/jimmunol.1001660
M3 - Article
C2 - 20889540
AN - SCOPUS:78149475086
SN - 0022-1767
VL - 185
SP - 5130
EP - 5139
JO - Journal of Immunology
JF - Journal of Immunology
IS - 9
ER -