Cytotoxicity evaluation and antimicrobial studies of starch capped water soluble copper nanoparticles

Mayur Valodkar, Puran Singh Rathore, Ravirajsinh N. Jadeja, Menaka Thounaojam, Ranjitsinh V. Devkar, Sonal Thakore

Research output: Contribution to journalArticlepeer-review

113 Scopus citations


Water soluble monodisperse copper nanoparticles of about 10. nm diameter were prepared by microwave irradiation using starch as green capping agent. The resulting Cu-starch conjugate were characterized by FTIR and energy dispersive X-ray analysis (EDX). The study confirmed the presence of copper embedded in polysaccharide matrix. The aqueous solution of starch capped copper nanoparticles (SCuNPs) exhibited excellent bactericidal action against both gram negative and gram positive bacteria. The . in vitro cytotoxicity evaluation of the nanoparticles was carried out using mouse embryonic fibroblast (3T3L1) cells by MTT cell viability assay, extracellular lactate dehydrogenase (LDH) release and dark field microscopy imaging. The capped nanoparticles exhibited cytotoxicity at much higher concentration compared to cupric ions. Minimum bactericidal concentration (MBC) of SCuNPs was well below the . in vitro cytotoxic concentration. Statistical analysis demonstrated . p<. 0.05 for significant results and . p>. 0.05 for non-significant ones as compared to untreated cells. The non-cytotoxic green Cu-starch conjugate offers a rational approach towards antimicrobial application and for integration to biomedical devices.

Original languageEnglish (US)
Pages (from-to)244-249
Number of pages6
JournalJournal of Hazardous Materials
StatePublished - Jan 30 2012
Externally publishedYes


  • Bactericidal
  • Copper nanoparticles
  • Cytotoxicity
  • Mouse embryonic fibroblast (3T3L1) cells

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution
  • Health, Toxicology and Mutagenesis


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