TY - JOUR
T1 - Cytotoxicity evaluation and antimicrobial studies of starch capped water soluble copper nanoparticles
AU - Valodkar, Mayur
AU - Rathore, Puran Singh
AU - Jadeja, Ravirajsinh N.
AU - Thounaojam, Menaka
AU - Devkar, Ranjitsinh V.
AU - Thakore, Sonal
N1 - Funding Information:
The authors are grateful to the U.G.C., New Delhi for financial assistance and Dr. Jayshree Pohnerkar and Mrs. Mukta Mohan, Department of Biochemistry, Faculty of Science, The M.S. University of Baroda, for antimicrobial studies.
PY - 2012/1/30
Y1 - 2012/1/30
N2 - Water soluble monodisperse copper nanoparticles of about 10. nm diameter were prepared by microwave irradiation using starch as green capping agent. The resulting Cu-starch conjugate were characterized by FTIR and energy dispersive X-ray analysis (EDX). The study confirmed the presence of copper embedded in polysaccharide matrix. The aqueous solution of starch capped copper nanoparticles (SCuNPs) exhibited excellent bactericidal action against both gram negative and gram positive bacteria. The . in vitro cytotoxicity evaluation of the nanoparticles was carried out using mouse embryonic fibroblast (3T3L1) cells by MTT cell viability assay, extracellular lactate dehydrogenase (LDH) release and dark field microscopy imaging. The capped nanoparticles exhibited cytotoxicity at much higher concentration compared to cupric ions. Minimum bactericidal concentration (MBC) of SCuNPs was well below the . in vitro cytotoxic concentration. Statistical analysis demonstrated . p<. 0.05 for significant results and . p>. 0.05 for non-significant ones as compared to untreated cells. The non-cytotoxic green Cu-starch conjugate offers a rational approach towards antimicrobial application and for integration to biomedical devices.
AB - Water soluble monodisperse copper nanoparticles of about 10. nm diameter were prepared by microwave irradiation using starch as green capping agent. The resulting Cu-starch conjugate were characterized by FTIR and energy dispersive X-ray analysis (EDX). The study confirmed the presence of copper embedded in polysaccharide matrix. The aqueous solution of starch capped copper nanoparticles (SCuNPs) exhibited excellent bactericidal action against both gram negative and gram positive bacteria. The . in vitro cytotoxicity evaluation of the nanoparticles was carried out using mouse embryonic fibroblast (3T3L1) cells by MTT cell viability assay, extracellular lactate dehydrogenase (LDH) release and dark field microscopy imaging. The capped nanoparticles exhibited cytotoxicity at much higher concentration compared to cupric ions. Minimum bactericidal concentration (MBC) of SCuNPs was well below the . in vitro cytotoxic concentration. Statistical analysis demonstrated . p<. 0.05 for significant results and . p>. 0.05 for non-significant ones as compared to untreated cells. The non-cytotoxic green Cu-starch conjugate offers a rational approach towards antimicrobial application and for integration to biomedical devices.
KW - Bactericidal
KW - Copper nanoparticles
KW - Cytotoxicity
KW - Mouse embryonic fibroblast (3T3L1) cells
UR - http://www.scopus.com/inward/record.url?scp=84855358484&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84855358484&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2011.11.077
DO - 10.1016/j.jhazmat.2011.11.077
M3 - Article
C2 - 22178277
AN - SCOPUS:84855358484
SN - 0304-3894
VL - 201-202
SP - 244
EP - 249
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
ER -