Defective antibody-mediated opsonisation of S. pneumoniae in high risk patients detected by flow cytometry

S. K. Obaro; D. C. Henderson; M. A. Monteil

doi:10.1016/0165-2478(95)02487-5

Defective antibody-mediated opsonisation of S. pneumoniae in high risk patients detected by flow cytometry

S. K. Obaro, D. C. Henderson, M. A. Monteil

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Invasive pneumococcal infection continues to be a significant cause of morbidity and mortality especially in patients with antibody deficiencies and disorders affecting the reticulo-endothelial system. Current recommendations for prophylaxis in these patients include immunization with 23-valent pneumococcal polysaccharide capsular vaccines. Post-immunization responses are commonly assessed by measuring serum antibody levels. However, there is no defined protective antibody range and this approach fails to determine the functional capacity of the antibodies. A simple, reproducible flow cytometric method of assessing the antibody-mediated opsonic activity against S. pneumoniae is described. This assay detected defective opsonic function in post-immunized at-risk patients who developed invasive pneumococcal infection.

Original language	English (US)
Pages (from-to)	83-89
Number of pages	7
Journal	Immunology Letters
Volume	49
Issue number	1-2
DOIs	https://doi.org/10.1016/0165-2478(95)02487-5
State	Published - Jan 1996
Externally published	Yes

Keywords

Flow cytometry
Functional antibody deficiency
Opsonisation
Phagocytosis
Streptococcus pneumoniae

ASJC Scopus subject areas

Immunology and Allergy
Immunology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0165-2478(95)02487-5

Cite this

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title = "Defective antibody-mediated opsonisation of S. pneumoniae in high risk patients detected by flow cytometry",

abstract = "Invasive pneumococcal infection continues to be a significant cause of morbidity and mortality especially in patients with antibody deficiencies and disorders affecting the reticulo-endothelial system. Current recommendations for prophylaxis in these patients include immunization with 23-valent pneumococcal polysaccharide capsular vaccines. Post-immunization responses are commonly assessed by measuring serum antibody levels. However, there is no defined protective antibody range and this approach fails to determine the functional capacity of the antibodies. A simple, reproducible flow cytometric method of assessing the antibody-mediated opsonic activity against S. pneumoniae is described. This assay detected defective opsonic function in post-immunized at-risk patients who developed invasive pneumococcal infection.",

keywords = "Flow cytometry, Functional antibody deficiency, Opsonisation, Phagocytosis, Streptococcus pneumoniae",

author = "Obaro, {S. K.} and Henderson, {D. C.} and Monteil, {M. A.}",

note = "Funding Information: The authors wish to thank Professor J.R. Hobbs for support and encouragement, MS Joyce Lynch for technical support and Mrs Rosemary Jenkinson for her excellent secretarial assistance. This study was made possible by a grant from the COGENT Trust.",

year = "1996",

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doi = "10.1016/0165-2478(95)02487-5",

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volume = "49",

pages = "83--89",

journal = "Immunology Letters",

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AU - Obaro, S. K.

AU - Henderson, D. C.

AU - Monteil, M. A.

N1 - Funding Information: The authors wish to thank Professor J.R. Hobbs for support and encouragement, MS Joyce Lynch for technical support and Mrs Rosemary Jenkinson for her excellent secretarial assistance. This study was made possible by a grant from the COGENT Trust.

PY - 1996/1

Y1 - 1996/1

N2 - Invasive pneumococcal infection continues to be a significant cause of morbidity and mortality especially in patients with antibody deficiencies and disorders affecting the reticulo-endothelial system. Current recommendations for prophylaxis in these patients include immunization with 23-valent pneumococcal polysaccharide capsular vaccines. Post-immunization responses are commonly assessed by measuring serum antibody levels. However, there is no defined protective antibody range and this approach fails to determine the functional capacity of the antibodies. A simple, reproducible flow cytometric method of assessing the antibody-mediated opsonic activity against S. pneumoniae is described. This assay detected defective opsonic function in post-immunized at-risk patients who developed invasive pneumococcal infection.

AB - Invasive pneumococcal infection continues to be a significant cause of morbidity and mortality especially in patients with antibody deficiencies and disorders affecting the reticulo-endothelial system. Current recommendations for prophylaxis in these patients include immunization with 23-valent pneumococcal polysaccharide capsular vaccines. Post-immunization responses are commonly assessed by measuring serum antibody levels. However, there is no defined protective antibody range and this approach fails to determine the functional capacity of the antibodies. A simple, reproducible flow cytometric method of assessing the antibody-mediated opsonic activity against S. pneumoniae is described. This assay detected defective opsonic function in post-immunized at-risk patients who developed invasive pneumococcal infection.

KW - Flow cytometry

KW - Functional antibody deficiency

KW - Opsonisation

KW - Phagocytosis

KW - Streptococcus pneumoniae

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Defective antibody-mediated opsonisation of S. pneumoniae in high risk patients detected by flow cytometry

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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