Abstract
The formation of hydroxyl radical in the post-ischemic reperfused heart was measured with high performance liquid chromatography and ultraviolet detection using salicylic acid. Hydroxyl radicals react with salicylic acid yielding 2,3- and 2,5-dihydroxybenzoic acid, which can be separated by the liquid chromatography. Isolated rat hearts were perfused with 1 mm salicylic acid and were subjected to 30 mins of global ischemia followed by aerobic or anaerobic reperfusion at 37°C. The effluent from the hearts was collected at various intervals, extracted with ether, and injected into the high performance liquid chromatography unit. 2,5-dihydroxybenzoic acid was present only after aerobic reperfusion and was not detected before ischemia. The liquid chromatography peak of 2,3-dihydroxybenzoic acid was too small for quantitation. The concentration of 2,5-dihydroxybenzoic acid was the highest within 300 s of reperfusion. 2,5-dihydroxybenzoic acid was not detected in the ischemic hearts during anaerobic reperfusion. In ischemic hearts perfused with mannitol, the amount of 2,5-dihydroxybenzoic acid after reperfusion was reduced. These data suggest that hydroxyl radicals are produced in the post-ischemic reperfused heart and that the present method is useful and reliable for the measurement of hydroxyl radicals in the heart.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 365-370 |
| Number of pages | 6 |
| Journal | Journal of molecular and cellular cardiology |
| Volume | 23 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 1991 |
| Externally published | Yes |
Keywords
- Hydroxyl radical
- Oxygen-free radicals
- Radical trapping agent
- Salicylic acid
- liquid chromatography
- post-ischemic reperfusion
ASJC Scopus subject areas
- Molecular Biology
- Cardiology and Cardiovascular Medicine