TY - JOUR
T1 - Determination of chlorpyrifos and its metabolites in rat blood using liquid chromatography/electrospray ionization tandem mass spectrometry
AU - Williamson, Leah N.
AU - Bartlett, Michael G.
AU - Terry, Alvin V
PY - 2007/1/1
Y1 - 2007/1/1
N2 - A method has been developed to quantify chlorpyrifos (O,O-diethyl O-[3,5,6,-trichloro-2-pyridyl] phosphorothionate) and its metabolites chlorpyrifos-oxon (O,O-diethyl-O[3,5,6,trichloro-2-pyridyl] phosphate) and TCP (3,5,6,-trichloro-2-pyridinol) in rat blood by liquid chromatography/ electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS). Rat blood was treated by liquid-liquid extraction and the analytes were separated by gradient elution on an Agilent Zorbax Extended-C8 column (2.0×150 mm, 5 μm). Chlorpyrifos and chlorpyrifos-oxon were detected in positive ion mode using multiple reaction monitoring (MRM). TCP was detected in negative ion mode using parent-to-parent transition monitoring.This method was validated and the specificity, linearity, limit of quantitation (LOQ), precision, accuracy, stability, and recoveries were determined. The method was then applied to determine the level of chlorpyrifos and its metabolites from rats exposed to a subcutaneous injection of 10 and 18 mg/kg.
AB - A method has been developed to quantify chlorpyrifos (O,O-diethyl O-[3,5,6,-trichloro-2-pyridyl] phosphorothionate) and its metabolites chlorpyrifos-oxon (O,O-diethyl-O[3,5,6,trichloro-2-pyridyl] phosphate) and TCP (3,5,6,-trichloro-2-pyridinol) in rat blood by liquid chromatography/ electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS). Rat blood was treated by liquid-liquid extraction and the analytes were separated by gradient elution on an Agilent Zorbax Extended-C8 column (2.0×150 mm, 5 μm). Chlorpyrifos and chlorpyrifos-oxon were detected in positive ion mode using multiple reaction monitoring (MRM). TCP was detected in negative ion mode using parent-to-parent transition monitoring.This method was validated and the specificity, linearity, limit of quantitation (LOQ), precision, accuracy, stability, and recoveries were determined. The method was then applied to determine the level of chlorpyrifos and its metabolites from rats exposed to a subcutaneous injection of 10 and 18 mg/kg.
KW - Chlorpyrifos
KW - Electrospray ionization
KW - HPLC
KW - Liquid-liquid extraction
KW - Organophosphate insecticides
KW - Tandem mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=33845670846&partnerID=8YFLogxK
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U2 - 10.1080/10826070601064573
DO - 10.1080/10826070601064573
M3 - Article
AN - SCOPUS:33845670846
SN - 1082-6076
VL - 30
SP - 273
EP - 285
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
IS - 2
ER -