Determination of human PDGF-B promoter binding nuclear protein by capillary electrophoresis

Xingjun Fan, J. Liu, Y. X. Jin, D. B. Wang

Research output: Contribution to journalArticlepeer-review

Abstract

With the use of 1.0% T, 0% C linear polyacrylamide as sieving matrix, 0.25 x TBE(Tris 89 mmol/L, boric acid 89 mmol/L, EDTA 2 mmol/L) as running buffer and 15 degrees C as column temperature, the human PDGF-B promoter binding nuclear protein can be determined within 50 min with good resolution. The results proved that there are two proteins having strong ability binding human PDGF-B promoter, which similar to that in slab gel electrophoresis. This technique can provide one of the rapid and accurate separation methods in the studying of the formation and repression behavior of DNA binding protein based on PDGF gene as the target.

Original languageEnglish (US)
Pages (from-to)170-172
Number of pages3
JournalSe pu = Chinese journal of chromatography / Zhongguo hua xue hui
Volume19
Issue number2
StatePublished - Mar 2001
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • General Chemical Engineering
  • Organic Chemistry
  • Electrochemistry

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