Determination of particle size distribution from tissue using light scattering spectroscopy with small source detector separations

Dheerendra Kashyap, Cole A. Giller, Hanli Liu

Research output: Contribution to journalConference articlepeer-review


The ability to retrieve particle size information from back scattering reflectance with a small source-detector separation would significantly enhance the potential for development of non-invasive and minimally invasive diagnostic techniques. We present a technique for inverse determination of particle size distribution and volume fractions and validate it with polystyrene microspheres. Two of monotonic, third-degree polynomial equations were fitted from Mie theory to relate wavelength exponent 'n' and particle radii. These two equations allow us to inversely estimate the particle size from the measured 'n' value. A genetic algorithm was applied to optimize the particle size distribution and volume fraction. The experimental setup consisted of a tungsten light, CCD spectrometer with a bifurcated optical fiber for light delivery and detection. The measurement system was calibrated with a reflectance standard; different sizes and volume fractions of the suspensions were chosen for measurements. The wavelength dependence of reduced scattering coefficient was derived from the measured reflectance. Polystyrene microsphere suspensions with diameters 0.43 - 2.00 μm were characterized using the developed algorithm. The results show a good agreement between the particle size retrieved by our algorithm and manufacturer's data, demonstrating a robust method for particle size determination using near infrared reflectance and small source-detector separation.

Original languageEnglish (US)
Article number70
Pages (from-to)359-365
Number of pages7
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
StatePublished - 2005
Externally publishedYes
EventOptical Tomography and Spectroscopy of Tissue VI - San Jose, CA, United States
Duration: Jan 23 2005Jan 26 2005


  • Cell size
  • Mitochondria
  • Nearinfrared spectroscopy
  • Particle size

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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