TY - JOUR
T1 - Development and comparative evaluation of a multiple-antigen RAST as a screening test for inhalant allergy
AU - Ownby, Dennis R.
AU - Anderson, John A.
AU - Jacobs, Gregory L.
AU - Homburger, Henry A.
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1984/4
Y1 - 1984/4
N2 - We have developed a modified in vitro test for IgE antibodies, the multi-RA ST, to detect antibodies of different allergen specificities simultaneously in a single tube. The multi-RAST is as sensitive for detecting low concentrations of individual IgE antibodies as the discrete RAST. We also evaluated the multi-RAST as a screening test for respiratory allergy to inhalant allergens in children by comparing the results of the multi-RAST performed by use of a mixture of SRP, TGP, and DF allergen-immunosorbents with the results of skin tests, discrete RAST tests for the same allergens, serum total IgE concentrations, and nasal smears for eosinophils in 100 children referred for allergic-disease evaluation. The results of the multi-RAST were more sensitive, specific, and efficient than the results of tests for serum IgE concentration and nasal eosinophils in establishing the diagnosis of inhalant allergy; the multi-RAST was the only diagnostic test that yielded results that were significantly associated with the clinician's impression of allergy. We conclude that the multi-RAST is a useful and cost-effective screening test for inhalant allergy in children.
AB - We have developed a modified in vitro test for IgE antibodies, the multi-RA ST, to detect antibodies of different allergen specificities simultaneously in a single tube. The multi-RAST is as sensitive for detecting low concentrations of individual IgE antibodies as the discrete RAST. We also evaluated the multi-RAST as a screening test for respiratory allergy to inhalant allergens in children by comparing the results of the multi-RAST performed by use of a mixture of SRP, TGP, and DF allergen-immunosorbents with the results of skin tests, discrete RAST tests for the same allergens, serum total IgE concentrations, and nasal smears for eosinophils in 100 children referred for allergic-disease evaluation. The results of the multi-RAST were more sensitive, specific, and efficient than the results of tests for serum IgE concentration and nasal eosinophils in establishing the diagnosis of inhalant allergy; the multi-RAST was the only diagnostic test that yielded results that were significantly associated with the clinician's impression of allergy. We conclude that the multi-RAST is a useful and cost-effective screening test for inhalant allergy in children.
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U2 - 10.1016/0091-6749(84)90356-7
DO - 10.1016/0091-6749(84)90356-7
M3 - Article
C2 - 6608549
AN - SCOPUS:0021280607
SN - 0091-6749
VL - 73
SP - 466
EP - 472
JO - The Journal of Allergy and Clinical Immunology
JF - The Journal of Allergy and Clinical Immunology
IS - 4
ER -