Development and evaluation of an enzyme-linked immunosorbent assay with recombinant SAG2 for diagnosis of Toxoplasma gondii infection in cats

X. Huang; X. Xuan; E. N. Kimbita; B. Battur; T. Miyazawa; S. Fukumoto; M. Mishima; L. H. Makala; H. Suzuki; C. Sugimoto; H. Nagasawa; K. Fujisaki; T. Mikami; I. Igarashi

doi:10.1645/0022-3395(2002)088[0804:DAEOAE]2.0.CO;2

Development and evaluation of an enzyme-linked immunosorbent assay with recombinant SAG2 for diagnosis of Toxoplasma gondii infection in cats

X. Huang, X. Xuan, E. N. Kimbita, B. Battur, T. Miyazawa, S. Fukumoto, M. Mishima, L. H. Makala, H. Suzuki, C. Sugimoto, H. Nagasawa, K. Fujisaki, T. Mikami, I. Igarashi

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32 Scopus citations

Abstract

Cats are pivotal in the transmission of Toxoplasma gondii. To develop a sensitive and specific serodiagnostic method for feline toxoplasmosis, surface antigen 2 (SAG2) of T. gondii was expressed in Escherichia coli and its diagnostic potential evaluated in an enzyme-linked immunosorbent assay (ELISA). The ELISA with recombinant SAG2 (rSAG2) was able to differentiate very clearly between sera from cats experimentally infected with T. gondii and sera from normal cats. Serum samples collected from domestic cats in Japan were investigated by the ELISA, and the results were compared with those of a commercially available latex agglutination test (LAT) kit. Of the 192 samples screened, 42 (21.9%) were positive by ELISA. Among the 42 ELISA-positive samples, 39 were positive by LAT. There was a significant correlation between ELISA and LAT titers. All the 150 ELISA-negative samples were negative by LAT. These results indicate that the ELISA with rSAG2 expressed in E. coli should be a useful method for detection of T. gondii infection in cats.

Original language	English (US)
Pages (from-to)	804-807
Number of pages	4
Journal	Journal of Parasitology
Volume	88
Issue number	4
DOIs	https://doi.org/10.1645/0022-3395(2002)088[0804:DAEOAE]2.0.CO;2
State	Published - 2002
Externally published	Yes

ASJC Scopus subject areas

Parasitology
Ecology, Evolution, Behavior and Systematics

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10.1645/0022-3395(2002)088[0804:DAEOAE]2.0.CO;2

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Huang, X., Xuan, X., Kimbita, E. N., Battur, B., Miyazawa, T., Fukumoto, S., Mishima, M., Makala, L. H., Suzuki, H., Sugimoto, C., Nagasawa, H., Fujisaki, K., Mikami, T., & Igarashi, I. (2002). Development and evaluation of an enzyme-linked immunosorbent assay with recombinant SAG2 for diagnosis of Toxoplasma gondii infection in cats. Journal of Parasitology, 88(4), 804-807. https://doi.org/10.1645/0022-3395(2002)088[0804:DAEOAE]2.0.CO;2

Huang, X, Xuan, X, Kimbita, EN, Battur, B, Miyazawa, T, Fukumoto, S, Mishima, M, Makala, LH, Suzuki, H, Sugimoto, C, Nagasawa, H, Fujisaki, K, Mikami, T & Igarashi, I 2002, 'Development and evaluation of an enzyme-linked immunosorbent assay with recombinant SAG2 for diagnosis of Toxoplasma gondii infection in cats', Journal of Parasitology, vol. 88, no. 4, pp. 804-807. https://doi.org/10.1645/0022-3395(2002)088[0804:DAEOAE]2.0.CO;2

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abstract = "Cats are pivotal in the transmission of Toxoplasma gondii. To develop a sensitive and specific serodiagnostic method for feline toxoplasmosis, surface antigen 2 (SAG2) of T. gondii was expressed in Escherichia coli and its diagnostic potential evaluated in an enzyme-linked immunosorbent assay (ELISA). The ELISA with recombinant SAG2 (rSAG2) was able to differentiate very clearly between sera from cats experimentally infected with T. gondii and sera from normal cats. Serum samples collected from domestic cats in Japan were investigated by the ELISA, and the results were compared with those of a commercially available latex agglutination test (LAT) kit. Of the 192 samples screened, 42 (21.9%) were positive by ELISA. Among the 42 ELISA-positive samples, 39 were positive by LAT. There was a significant correlation between ELISA and LAT titers. All the 150 ELISA-negative samples were negative by LAT. These results indicate that the ELISA with rSAG2 expressed in E. coli should be a useful method for detection of T. gondii infection in cats.",

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AU - Huang, X.

AU - Xuan, X.

AU - Kimbita, E. N.

AU - Battur, B.

AU - Miyazawa, T.

AU - Fukumoto, S.

AU - Mishima, M.

AU - Makala, L. H.

AU - Suzuki, H.

AU - Sugimoto, C.

AU - Nagasawa, H.

AU - Fujisaki, K.

AU - Mikami, T.

AU - Igarashi, I.

PY - 2002

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Development and evaluation of an enzyme-linked immunosorbent assay with recombinant SAG2 for diagnosis of Toxoplasma gondii infection in cats

Abstract

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