Development of an Antibacterial Dentin Adhesive

Stephanie R. Lopes, Amanda G.N. Matuda, Raquel P. Campos, Ana Paula V.P. Mafetano, Ana Helena M. Barnabe, Gabriela S. Chagas, Daphne C. Barcellos, Li Na Niu, Franklin R. Tay, Cesar R. Pucci

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Nisin is a peptide that possesses potent antibacterial properties. This study evaluated the antibacterial activity of a nisin-doped adhesive against Streptococcus mutans, as well as its degree of conversion and microtensile bond strength (µTBS) to dentin. Nisin was added to the adhesive Adper Single Bond 2 (3M ESPE), resulting in four groups: Control Group (Single Bond 2); Group 1% (1 wt% nisin-incorporated), Group 3% (3 wt% nisin-incorporated) and Group 5% (5 wt% nisin-incorporated). Antibacterial activity against S. mutans was evaluated using colony-forming unit counts (CFU). The degree of conversion was tested using FTIR. Forty human teeth were restored for µTBS evaluation. Data were statistically analyzed with ANOVA and Tukey tests at α = 0.05. The nisin-doped adhesives, for all concentrations, exhibited a significant inhibition of the growth of S. mutans (p < 0.05); Incorporation of 5% and 3% nisin decreased the degree of conversion of the adhesive (p < 0.05). The µTBS (in MPa): Control Group—38.3 ± 2.3A, Group 1%—35.6 ± 2.1A, Group 3%—27.1 ± 1.6B and Group 5%—22.3 ± 1.0C. Nisin-doped adhesives exerted a bactericidal effect on S. mutans. The µTBS and degree of conversion of adhesive were not affected after incorporation of 1% nisin.

Original languageEnglish (US)
Article number2502
Issue number12
StatePublished - Jun 1 2022


  • antibacterial activity
  • dentin
  • etch-and-rinse adhesive
  • nisin

ASJC Scopus subject areas

  • General Chemistry
  • Polymers and Plastics


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