Dietary fish oil is antihypertrophic but does not enhance postischemic myocardial function in female mice

Catherine E. Huggins, Claire L. Curl, Ruchi Patel, Peter L. McLennan, Mandy L. Theiss, Thierry Pedrazzini, Salvatore Pepe, Lea M.D. Delbridge

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Clinically and experimentally, a case for omega-3 polyunsaturated fatty acid (PUFA) cardioprotection in females has not been clearly established. The goal of this study was to investigate whether dietary omega-3 PUFA supplementation could provide ischemic protection in female mice with an underlying genetic predisposition to cardiac hypertrophy. Mature female transgenic mice (TG) with cardiac-specific overexpression of angiotensinogen that develop normotensive cardiac hypertrophy and littermate wild-type (WT) mice were fed a fish oil-derived diet (FO) or PUFA-matched control diet (CTR) for 4 wk. Myocardial membrane lipids, ex vivo cardiac performance (intraventricular balloon) after global no-flow ischemia and reperfusion (15/30 min), and reperfusion arrhythmia incidence were assessed. FO diet suppressed cardiac growth by 5% and 10% in WT and TG, respectively (P > 0.001). The extent of mechanical recovery [rate- pressure product (RPP) = beats/min X mmHg] of FO-fed WT and TG hearts was similar (50 ± 7% vs. 45 ± 12%, 30 min reperfusion), and this was not significantly different from CTR-fed WT or TG. To evaluate whether systemic estrogen was masking a protective effect of the FO diet, the responses of ovariectomized (OVX) WT and TG mice to FO dietary intervention were assessed. The extent of mechanical recovery of FO-fed OVX WT and TG (RPP, 50 ± 4% vs. 64 ± 8%) was not enhanced compared with CTR-fed mice (RPP, 60 ± 11% vs. 80 ± 8%, P = 0.335). Dietary FO did not suppress the incidence of reperfusion arrhythmias in WT or TG hearts (ovary-intact mice or OVX). Our findings indicate a lack of cardioprotective effect of dietary FO in females, determined by assessment of mechanical and arrhythmic activity postischemia in a murine ex vivo heart model.polyunsaturated fatty acids; ischemia-reperfusion; Langendorff perfused hearts

Original languageEnglish (US)
Pages (from-to)H957-H966
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number4
StatePublished - Apr 2009
Externally publishedYes


  • Ischemia-reperfusion
  • Langendorff perfused hearts
  • polyunsaturated fatty acids

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


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