Differential DNA methylation of gene promoters in small B-cell lymphomas

Juyuan Guo, Matthias Burger, Inko Nimmrich, Sabine Maier, Evelyne Becker, Buelent Genc, Dieter Duff, Farahnaz Rahmatpanah, Rebecca Chitma-Matsiga, Huidong Shi, Kurt Berlin, Tim H.M. Huang, Charks W. Caldwell

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Improved care of patients with small B-cell lymphomas (SBCLs) is likely to result from the ongoing discovery of molecular markers that better define these malignant neoplasms. We identified multiple gene loci whose DNA methylation patterns differed between 3 types of SBCL: B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma, mantle cell lymphoma, and grades I and II follicular lymphoma. This analysis was performed using an oligonucleotide microarray that allowed determination of the DNA methylation status of 156 loci in 38 genes. Combined bisulfite restriction analysis and methylation-specific polymerase chain reaction were used to validate the differential methylation of 6 of these genes. By using non-Hodgkin lymphoma cell lines as models, these genes were examined further for methylation and gene expression relationships. This study illustrates nonrandom epigenetic alterations in SBCLs that seem to preferentially involve lymphomas of germinal center derivation.

Original languageEnglish (US)
Pages (from-to)430-439
Number of pages10
JournalAmerican Journal of Clinical Pathology
Issue number3
StatePublished - Sep 2005
Externally publishedYes


  • DNA methylation
  • Epigenetics
  • Non-Hodgkin lymphoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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