Abstract
To determine whether α smooth muscle cell actin (αSMCA) is expressed in human fibroblasts isolated from normal peritoneal and adhesion tissues. Prospective, experimental study. University medical center. Five patients undergoing laparotomy for adhesiolysis for pelvic pain. Primary culture of fibroblasts from both peritoneum and adhesion tissues. Immunohistochemistry techniques were used to determine whether αSMCA was present in normal peritoneal and adhesion fibroblasts from the same patients. Cultured fibroblasts from all tissues were also fixed on slides and stained with αSMCA monoclonal antibody labeled with immunofluorescence. Alpha SMCA protein was present in very low levels in the cytoplasm of normal peritoneal fibroblasts from all five subjects, as compared with high levels present in adhesion fibroblasts from the same patients. Adhesion fibroblasts develop a specific phenotype, an adhesion phenotype, which in part is characterized by the over-expression of αSMCA. The over-expression of αSMCA in adhesion fibroblasts indicates a possible response to peritoneal injury. Regulation of αSMCA might alter peritoneal healing and might provide the opportunity to reduce postoperative adhesion development.
Original language | English (US) |
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Pages (from-to) | 1188-1192 |
Number of pages | 5 |
Journal | Fertility and sterility |
Volume | 82 |
Issue number | SUPPL. 3 |
DOIs | |
State | Published - Oct 2004 |
Externally published | Yes |
Keywords
- Fibroblasts
- adhesion
- immunohistochemistry
- peritoneum
- αSMCA
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynecology