TY - JOUR
T1 - Differential expression of TGF-β1 and TGF-β3 in serosal tissues of human intraperitoneal organs and peritoneal adhesions
AU - Chegini, Nasser
AU - Kotseos, Kristina
AU - Zhao, Yong
AU - Bennett, Barbara
AU - McLean, Frederick W.
AU - Diamond, Michael Peter
AU - Holmdahl, Lina
AU - Burns, James
AU - Williams, R. Stan
AU - Ma, Chunfeng
AU - Patel, Alpa
AU - Goldberg, Eugene
AU - Peck, Lynn
AU - Saed, Ghassen
AU - Leach, Richard
AU - Collins, Karen
AU - Yealin, Frank
AU - Svinvich, David
AU - Serokin, Yoram
AU - Falk, Peter
AU - Ivarsson, Marie Louise
AU - Hedgren, Maria
AU - Bergstrom, Maria
AU - Palmgren, Ingrid
AU - Burns, Jim
AU - Skinner, Kevin
AU - Nickerson, Cindy
PY - 2001
Y1 - 2001
N2 - Elevated local expression of transforming growth factor (TGF-β) has been associated with increased incidence of peritoneal adhesion formation. In this study we determine whether differences in basal expression of TGF-β in serosal tissue of peritoneal organs correlate with incidence of adhesion formation. Serosal tissue of parietal peritoneum, uterus, oviduct, ovary, omentum, large and small bowels as well as adhesions, skin, fascia, subcutaneous tissue, peritoneal fluid and serum were collected from 57 subjects with/without adhesions who were undergoing abdominal/pelvic surgery. To determine TGF-β1 and TGF-β3 mRNA and protein expression, total RNA and protein were isolated from these tissues and along with the fluids, subjected to quantitative RT-PCR and enzyme-linked immunosorbent assay (ELISA) respectively. Tissue sections were immunostained for TGF-β1 and TGF-β3 protein. We found that TGF-β1 and TGF-β3 mRNA and protein are expressed in these tissues and present in peritoneal fluids and serum, with considerable variations in level of their expression. Comparatively, there was more variation in TGF-β1 than TGF-β3 expression without age or gender relation. Adhesions express a significantly higher TGF-β1 mRNA and have the highest TGF-β1:TGF-β3 ratio, with lowest concentrations and ratio detected in omentum, small and large bowels; in contrast uterus expresses higher TGF-β3, with lowest concentrations detected in subcutaneous tissue and large bowels (P < 0.05). A similar trend was also observed for total (active + latent) TGF-β1 protein expression, with low active TGF-β1 that was not significantly different among the tissue extracts and fluids. However, the lowest active:total TGF-β1 ratio was found in adhesions and ovary. In subjects with adhesions, the adhesions express significantly more TGF-β1 compared to parietal peritoneum (P < 0.05). Immunoreactive TGF-β1 and TGF-β3 protein were present in various cell types in these tissues with intensity reflecting their mRNA and protein expression. In conclusion, we provided evidence that serosal tissue of various peritoneal organs and adhesions express TGF-β1 and TGF-β3. Since TGF-β is expressed differently in these tissues and tissue injury often alters the expression of TGF-β, we propose that tissues with a higher basal expression of TGF-β may become predisposed to develop more adhesions compared to others.
AB - Elevated local expression of transforming growth factor (TGF-β) has been associated with increased incidence of peritoneal adhesion formation. In this study we determine whether differences in basal expression of TGF-β in serosal tissue of peritoneal organs correlate with incidence of adhesion formation. Serosal tissue of parietal peritoneum, uterus, oviduct, ovary, omentum, large and small bowels as well as adhesions, skin, fascia, subcutaneous tissue, peritoneal fluid and serum were collected from 57 subjects with/without adhesions who were undergoing abdominal/pelvic surgery. To determine TGF-β1 and TGF-β3 mRNA and protein expression, total RNA and protein were isolated from these tissues and along with the fluids, subjected to quantitative RT-PCR and enzyme-linked immunosorbent assay (ELISA) respectively. Tissue sections were immunostained for TGF-β1 and TGF-β3 protein. We found that TGF-β1 and TGF-β3 mRNA and protein are expressed in these tissues and present in peritoneal fluids and serum, with considerable variations in level of their expression. Comparatively, there was more variation in TGF-β1 than TGF-β3 expression without age or gender relation. Adhesions express a significantly higher TGF-β1 mRNA and have the highest TGF-β1:TGF-β3 ratio, with lowest concentrations and ratio detected in omentum, small and large bowels; in contrast uterus expresses higher TGF-β3, with lowest concentrations detected in subcutaneous tissue and large bowels (P < 0.05). A similar trend was also observed for total (active + latent) TGF-β1 protein expression, with low active TGF-β1 that was not significantly different among the tissue extracts and fluids. However, the lowest active:total TGF-β1 ratio was found in adhesions and ovary. In subjects with adhesions, the adhesions express significantly more TGF-β1 compared to parietal peritoneum (P < 0.05). Immunoreactive TGF-β1 and TGF-β3 protein were present in various cell types in these tissues with intensity reflecting their mRNA and protein expression. In conclusion, we provided evidence that serosal tissue of various peritoneal organs and adhesions express TGF-β1 and TGF-β3. Since TGF-β is expressed differently in these tissues and tissue injury often alters the expression of TGF-β, we propose that tissues with a higher basal expression of TGF-β may become predisposed to develop more adhesions compared to others.
KW - Fibrotic disorder
KW - Inflammatory immune response
KW - Peritoneal adhesion
KW - TGF-β1
KW - TGF-β3
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M3 - Article
C2 - 11387308
AN - SCOPUS:0034964898
SN - 0268-1161
VL - 16
SP - 1291
EP - 1300
JO - Human Reproduction
JF - Human Reproduction
IS - 6
ER -