@inproceedings{da743d3b77fb4564ac63876ab09ee5e4,
title = "Dispersion functions and factors that determine resolution for DNA sequencing by gel electrophoresis",
abstract = "The number of bases that can be read in a single run by a DNA sequencing instrument that detects fluorophore labeled DNA arriving at a 'finish-line' located a fixed distance from the starting wells is influenced by numerous parameters. Strategies for improving the length-of- read of a DNA sequencer can be based on quantitative models of the separation of DNA by gel electrophoresis. The dispersion function of the electrophoretic system - the relationship between molecular contour length and time of arrival at the detector - is useful in characterizing the performance of a DNA sequencer. We adapted analytical representations of dispersion functions, originally developed for snapshot imaging of DNA gels, (samples electrophoresed for constant time), to finish-line imaging, and demonstrated that a logistic- type function with non-integral exponent is required to describe the experimental data. We use this dispersion function to determine the resolution length and resolving power of a LI-COR DNA sequencing system and a custom built capillary gel electrophoresis system, and discuss the factors that presently limit the number of bases that can be determined reliably in a single sequencing run.",
author = "Sutherland, {John C.} and Reynolds, {Kiley J.} and Fisk, {David J.}",
year = "1996",
language = "English (US)",
isbn = "0819420549",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
pages = "326--340",
editor = "Cohn, {Gerald E.} and Soper, {Steven A.} and C.H.W. Chen",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",
note = "Ultrasensitive Biochemical Diagnostics ; Conference date: 31-01-1996 Through 02-02-1996",
}