TY - JOUR
T1 - Display of green fluorescent protein on Escherichia coli cell surface
AU - Shi, Huidong
AU - Wen Su, Wei
N1 - Funding Information:
We would like to thank Drs. G. Geogiou and S. Falkow for providing us with plasmids pTX215 and pGFPmut2. We are grateful to Drs. J.A. Benitez and M.Y. Zhou for helpful discussions and to Dr. T. Randy and Ms. J. Wagner for their technical assistance in electron microscopy and image processing. This work was supported in part by a grant from the University of Missouri Research Board.
PY - 2001/1/2
Y1 - 2001/1/2
N2 - In this study, expression of green fluorescence protein (GFP) on the external surface of Escherichia coli was achieved by construction of a fusion protein between Lpp-OmpA hybrid and a GFP variant, GFPmut2. The GFP was fused in frame to the carboxyl-terminus of Lpp-OmpA fusion previously shown to direct various other heterologous proteins to E. coli cell surface. Western blot analysis of membrane fractions identified the Lpp-OmpA-GFP fusion protein with the expected size (43 kDa). Immunofluorescence microscopy, immunoelectron microscopy, protease and extracellular pH sensitivity assays further confirmed that GFP is anchored on the outer membrane. The GFP displayed on the E. coli outer surface retained its fluorescence and was not susceptible to the indigenous outer membrane protease OmpT even though there are two putative OmpT proteolytic sites present in GFP. Optimization of the expression conditions was conducted using fluorometry, eliminating cumbersome immuno-labeling procedures. Surface-displayed GFP could be used in a variety of applications including screening of polypeptide libraries, development of live vaccines, construction of whole cell allosteric biosensors, and signal transduction studies. (C) 2001 Elsevier Science Inc.
AB - In this study, expression of green fluorescence protein (GFP) on the external surface of Escherichia coli was achieved by construction of a fusion protein between Lpp-OmpA hybrid and a GFP variant, GFPmut2. The GFP was fused in frame to the carboxyl-terminus of Lpp-OmpA fusion previously shown to direct various other heterologous proteins to E. coli cell surface. Western blot analysis of membrane fractions identified the Lpp-OmpA-GFP fusion protein with the expected size (43 kDa). Immunofluorescence microscopy, immunoelectron microscopy, protease and extracellular pH sensitivity assays further confirmed that GFP is anchored on the outer membrane. The GFP displayed on the E. coli outer surface retained its fluorescence and was not susceptible to the indigenous outer membrane protease OmpT even though there are two putative OmpT proteolytic sites present in GFP. Optimization of the expression conditions was conducted using fluorometry, eliminating cumbersome immuno-labeling procedures. Surface-displayed GFP could be used in a variety of applications including screening of polypeptide libraries, development of live vaccines, construction of whole cell allosteric biosensors, and signal transduction studies. (C) 2001 Elsevier Science Inc.
KW - Escherichia coli
KW - Green fluorescence protein
KW - Protein display
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U2 - 10.1016/S0141-0229(00)00281-7
DO - 10.1016/S0141-0229(00)00281-7
M3 - Article
AN - SCOPUS:0035793148
SN - 0141-0229
VL - 28
SP - 25
EP - 34
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
IS - 1
ER -