TY - JOUR
T1 - Distinct roles in lymphoid organogenesis for lymphotoxins α and β revealed in lymphotoxin β-deficient mice
AU - Koni, Pandelakis A.
AU - Sacca, Rosalba
AU - Lawton, Pornsri
AU - Browning, Jeffrey L.
AU - Ruddle, Nancy H.
AU - Flavell, Richard A.
N1 - Funding Information:
Correspondence should be addressed to R. A. F., N. H. R., or J. L. B. We are indebted to Jacques Peschon (Immunex) for allowing us the use of double TNFR–deficient mice, kindly provided by Gregory Geba (Yale University). We also thank David Chaplin (Washington University, St. Louis) for LTα-deficient mice; Mark Shlomchik, Lynn Hannum, and Ann Haberman for FDC-M1 and for guidance in NP-hapten immunizations and germinal center staining; Irene Dougas Sizing, Mohammad Zafari, and Chris Benjamin (Biogen) for anti-LTα and anti-LTβ antibodies; Cheryl Bergman for cytotoxicity assays; Jianchao Xu for the LTβ genomic clone; Paul Rennert for MLN B and T immunofluorescence; and Mary Margaret Cole, Frank Wilson, Linda Evangelisti, Debbie Butkus, and Cindy Hughes for technical assistance. This work was supported by the Howard Hughes Medical Institute (R. A. F.), a Human Frontier Science Program Long Term Fellowship (P. A. K.), a National Institutes of Health (NIH) Office of Research on Women's Health/Scientists Re-Entry Program Fellowship (R. S.), NIH R01 CA16885 (N. H. R.), NIH R01 AI34404 (N. H. R.), and the National Multiple Sclerosis Society RG2394 (N. H. R.).
PY - 1997/4/1
Y1 - 1997/4/1
N2 - Lymphotoxin α (LTα)-deficient mice revealed critical roles for LTα in lymphoid organogenesis, but it is not clear whether LTα functions through an LTα homotrimer (LTα3 or LTα/β heterotrimers. We generated LTβ-deficient mice and found them to lack Peyer's patches, peripheral lymph nodes, splenic germinal centers, and follicular dendritic cells. Unlike LTα-deficient mice, LTβ-deficient mice had cervical and mesenteric lymph nodes. Furthermore, the mesenteric lymph nodes had germinal center-like regions, although these structures appeared to lack follicular dendritic cells. The absence of cervical and mesenteric lymph nodes in LTα-deficient mice, and yet their presence in LTβ-deficient mice and in mice deficient in tumor necrosis factor receptor types I and II, suggest that LTα3 may signal via an as yet unidentified receptor.
AB - Lymphotoxin α (LTα)-deficient mice revealed critical roles for LTα in lymphoid organogenesis, but it is not clear whether LTα functions through an LTα homotrimer (LTα3 or LTα/β heterotrimers. We generated LTβ-deficient mice and found them to lack Peyer's patches, peripheral lymph nodes, splenic germinal centers, and follicular dendritic cells. Unlike LTα-deficient mice, LTβ-deficient mice had cervical and mesenteric lymph nodes. Furthermore, the mesenteric lymph nodes had germinal center-like regions, although these structures appeared to lack follicular dendritic cells. The absence of cervical and mesenteric lymph nodes in LTα-deficient mice, and yet their presence in LTβ-deficient mice and in mice deficient in tumor necrosis factor receptor types I and II, suggest that LTα3 may signal via an as yet unidentified receptor.
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U2 - 10.1016/S1074-7613(00)80292-7
DO - 10.1016/S1074-7613(00)80292-7
M3 - Article
C2 - 9133428
AN - SCOPUS:0030917003
SN - 1074-7613
VL - 6
SP - 491
EP - 500
JO - Immunity
JF - Immunity
IS - 4
ER -