DNA demethylation in retinal neurocytes contributes to the upregulation of DNA repair protein, Ku80

Jing Zhuang; Yiming Ye; Xuan Liu; Fan Li; Xueke Pan; Zhao Chen; Huihui Luo; Yihong Ge; Jian Ge; Joseph Michael Kaminski; Keming Yu

doi:10.1097/WNR.0b013e328336ee7e

DNA demethylation in retinal neurocytes contributes to the upregulation of DNA repair protein, Ku80

Jing Zhuang, Yiming Ye, Xuan Liu, Fan Li, Xueke Pan, Zhao Chen, Huihui Luo, Yihong Ge, Jian Ge, Joseph Michael Kaminski, Keming Yu

Radiation Oncology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Ku80 plays a critical role in DNA double strand breaks repair. However, Ku80 is silenced in mature neurocytes. In this study, the mechanism of Ku80 silencing and its role in DNA double strand break repair in retinal neurocytes was investigated. Our data show that Ku80 expression is activated in primary cultured retinal neurocytes after treatment with 5-azacytidine in vitro, whereas methylation of -179 bp in Ku80 promoter induces Ku80 silencing in retinal neurocytes. Ku80 reactivation in retinal neurocytes by 5-azacytidine enhances DNA integrity after treatment with H₂O₂. Therefore, our data suggest Ku80 might be a target for reactivation to increase retinal neuronal DNA repair.

Original language	English (US)
Pages (from-to)	282-286
Number of pages	5
Journal	NeuroReport
Volume	21
Issue number	4
DOIs	https://doi.org/10.1097/WNR.0b013e328336ee7e
State	Published - Mar 2010

Keywords

DNA repair
Ku80
Methylation
Nonhomologues end joining

ASJC Scopus subject areas

General Neuroscience

Access to Document

10.1097/WNR.0b013e328336ee7e

Cite this

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title = "DNA demethylation in retinal neurocytes contributes to the upregulation of DNA repair protein, Ku80",

abstract = "Ku80 plays a critical role in DNA double strand breaks repair. However, Ku80 is silenced in mature neurocytes. In this study, the mechanism of Ku80 silencing and its role in DNA double strand break repair in retinal neurocytes was investigated. Our data show that Ku80 expression is activated in primary cultured retinal neurocytes after treatment with 5-azacytidine in vitro, whereas methylation of -179 bp in Ku80 promoter induces Ku80 silencing in retinal neurocytes. Ku80 reactivation in retinal neurocytes by 5-azacytidine enhances DNA integrity after treatment with H2O2. Therefore, our data suggest Ku80 might be a target for reactivation to increase retinal neuronal DNA repair.",

keywords = "DNA repair, Ku80, Methylation, Nonhomologues end joining",

author = "Jing Zhuang and Yiming Ye and Xuan Liu and Fan Li and Xueke Pan and Zhao Chen and Huihui Luo and Yihong Ge and Jian Ge and Kaminski, {Joseph Michael} and Keming Yu",

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doi = "10.1097/WNR.0b013e328336ee7e",

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T1 - DNA demethylation in retinal neurocytes contributes to the upregulation of DNA repair protein, Ku80

AU - Zhuang, Jing

AU - Ye, Yiming

AU - Liu, Xuan

AU - Li, Fan

AU - Pan, Xueke

AU - Chen, Zhao

AU - Luo, Huihui

AU - Ge, Yihong

AU - Ge, Jian

AU - Kaminski, Joseph Michael

AU - Yu, Keming

PY - 2010/3

Y1 - 2010/3

N2 - Ku80 plays a critical role in DNA double strand breaks repair. However, Ku80 is silenced in mature neurocytes. In this study, the mechanism of Ku80 silencing and its role in DNA double strand break repair in retinal neurocytes was investigated. Our data show that Ku80 expression is activated in primary cultured retinal neurocytes after treatment with 5-azacytidine in vitro, whereas methylation of -179 bp in Ku80 promoter induces Ku80 silencing in retinal neurocytes. Ku80 reactivation in retinal neurocytes by 5-azacytidine enhances DNA integrity after treatment with H2O2. Therefore, our data suggest Ku80 might be a target for reactivation to increase retinal neuronal DNA repair.

AB - Ku80 plays a critical role in DNA double strand breaks repair. However, Ku80 is silenced in mature neurocytes. In this study, the mechanism of Ku80 silencing and its role in DNA double strand break repair in retinal neurocytes was investigated. Our data show that Ku80 expression is activated in primary cultured retinal neurocytes after treatment with 5-azacytidine in vitro, whereas methylation of -179 bp in Ku80 promoter induces Ku80 silencing in retinal neurocytes. Ku80 reactivation in retinal neurocytes by 5-azacytidine enhances DNA integrity after treatment with H2O2. Therefore, our data suggest Ku80 might be a target for reactivation to increase retinal neuronal DNA repair.

KW - DNA repair

KW - Ku80

KW - Methylation

KW - Nonhomologues end joining

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DNA demethylation in retinal neurocytes contributes to the upregulation of DNA repair protein, Ku80

Abstract

Keywords

ASJC Scopus subject areas

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