DNA epitope vaccine containing complement component C3d enhances anti-amyloid-β antibody production and polarizes the immune response towards a Th2 phenotype

Nina Movsesyan; Mikayel Mkrtichyan; Irina Petrushina; Ted M. Ross; David H. Cribbs; Michael G. Agadjanyan; Anahit Ghochikyan

doi:10.1016/j.jneuroim.2008.08.016

DNA epitope vaccine containing complement component C3d enhances anti-amyloid-β antibody production and polarizes the immune response towards a Th2 phenotype

Nina Movsesyan, Mikayel Mkrtichyan, Irina Petrushina, Ted M. Ross, David H. Cribbs, Michael G. Agadjanyan, Anahit Ghochikyan

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

We have engineered a DNA epitope vaccine that expresses 3 self-B cell epitopes of Aβ₄₂ (3Aβ_1-11), a non-self T helper (Th) cell epitope (PADRE), and 3 copies of C3d (3C3d), a component of complement as a molecular adjuvant, designed to safely reduce CNS Aβ. Immunization of mice with 3Aβ_1-11-PADRE epitope vaccine alone generated only moderate levels of anti-Aβ antibodies and a pro-inflammatory T helper (Th1 phenotype) cellular immune response. However, the addition of 3C3d to the vaccine construct significantly augmented the anti-Aβ humoral immune response and, importantly, shifted the cellular immune response towards the potentially safer anti-inflammatory Th2 phenotype.

Original language	English (US)
Pages (from-to)	57-63
Number of pages	7
Journal	Journal of Neuroimmunology
Volume	205
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2008.08.016
State	Published - Dec 15 2008
Externally published	Yes

Keywords

AN-1792
Alzheimer's disease
Amyloid-beta
DNA vaccine
Immunotherapy
Molecular adjuvant

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jneuroim.2008.08.016

Cite this

Movsesyan, N., Mkrtichyan, M., Petrushina, I., Ross, T. M., Cribbs, D. H., Agadjanyan, M. G., & Ghochikyan, A. (2008). DNA epitope vaccine containing complement component C3d enhances anti-amyloid-β antibody production and polarizes the immune response towards a Th2 phenotype. Journal of Neuroimmunology, 205(1-2), 57-63. https://doi.org/10.1016/j.jneuroim.2008.08.016

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title = "DNA epitope vaccine containing complement component C3d enhances anti-amyloid-β antibody production and polarizes the immune response towards a Th2 phenotype",

abstract = "We have engineered a DNA epitope vaccine that expresses 3 self-B cell epitopes of Aβ42 (3Aβ1-11), a non-self T helper (Th) cell epitope (PADRE), and 3 copies of C3d (3C3d), a component of complement as a molecular adjuvant, designed to safely reduce CNS Aβ. Immunization of mice with 3Aβ1-11-PADRE epitope vaccine alone generated only moderate levels of anti-Aβ antibodies and a pro-inflammatory T helper (Th1 phenotype) cellular immune response. However, the addition of 3C3d to the vaccine construct significantly augmented the anti-Aβ humoral immune response and, importantly, shifted the cellular immune response towards the potentially safer anti-inflammatory Th2 phenotype.",

keywords = "AN-1792, Alzheimer's disease, Amyloid-beta, DNA vaccine, Immunotherapy, Molecular adjuvant",

author = "Nina Movsesyan and Mikayel Mkrtichyan and Irina Petrushina and Ross, {Ted M.} and Cribbs, {David H.} and Agadjanyan, {Michael G.} and Anahit Ghochikyan",

note = "Funding Information: This work was supported by funding from NIH: AG 20241 (DHC), NS 50895 (DHC), AG 00538 (DHC), NS 057395 (MGA), Alzheimer's Association IIRG 036279 (AG). NM was supported by NIA training grant AG00096.",

year = "2008",

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doi = "10.1016/j.jneuroim.2008.08.016",

language = "English (US)",

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AU - Movsesyan, Nina

AU - Mkrtichyan, Mikayel

AU - Petrushina, Irina

AU - Ross, Ted M.

AU - Cribbs, David H.

AU - Agadjanyan, Michael G.

AU - Ghochikyan, Anahit

N1 - Funding Information: This work was supported by funding from NIH: AG 20241 (DHC), NS 50895 (DHC), AG 00538 (DHC), NS 057395 (MGA), Alzheimer's Association IIRG 036279 (AG). NM was supported by NIA training grant AG00096.

PY - 2008/12/15

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N2 - We have engineered a DNA epitope vaccine that expresses 3 self-B cell epitopes of Aβ42 (3Aβ1-11), a non-self T helper (Th) cell epitope (PADRE), and 3 copies of C3d (3C3d), a component of complement as a molecular adjuvant, designed to safely reduce CNS Aβ. Immunization of mice with 3Aβ1-11-PADRE epitope vaccine alone generated only moderate levels of anti-Aβ antibodies and a pro-inflammatory T helper (Th1 phenotype) cellular immune response. However, the addition of 3C3d to the vaccine construct significantly augmented the anti-Aβ humoral immune response and, importantly, shifted the cellular immune response towards the potentially safer anti-inflammatory Th2 phenotype.

AB - We have engineered a DNA epitope vaccine that expresses 3 self-B cell epitopes of Aβ42 (3Aβ1-11), a non-self T helper (Th) cell epitope (PADRE), and 3 copies of C3d (3C3d), a component of complement as a molecular adjuvant, designed to safely reduce CNS Aβ. Immunization of mice with 3Aβ1-11-PADRE epitope vaccine alone generated only moderate levels of anti-Aβ antibodies and a pro-inflammatory T helper (Th1 phenotype) cellular immune response. However, the addition of 3C3d to the vaccine construct significantly augmented the anti-Aβ humoral immune response and, importantly, shifted the cellular immune response towards the potentially safer anti-inflammatory Th2 phenotype.

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KW - Alzheimer's disease

KW - Amyloid-beta

KW - DNA vaccine

KW - Immunotherapy

KW - Molecular adjuvant

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DNA epitope vaccine containing complement component C3d enhances anti-amyloid-β antibody production and polarizes the immune response towards a Th2 phenotype

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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