DNA from various tissues of far eastern larches and its applicability for RAPD assay

I. Yu Sazonova; M. M. Kozyrenko; E. V. Artyukova; G. D. Reunova; Y. N. Zhuravlev

doi:10.1023/A:1009479319111

DNA from various tissues of far eastern larches and its applicability for RAPD assay

I. Yu Sazonova, M. M. Kozyrenko, E. V. Artyukova, G. D. Reunova, Y. N. Zhuravlev

Medicine

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We studied applicability of RAPD assay for investigating genetic variability of far eastern larches. Techniques to isolate DNA from larch vegetative organs (needles, young shoots and stems) and megagametophytes were selected, and conditions of polymerase chain reaction were optimized. Fifty out of 84 random primers proved efficient, and 22 and 12 primers initiated weak and no synthesis, respectively. The tested primers worked efficiently on the DNA isolated from both diploid and haploid tissues.

Original language	English (US)
Pages (from-to)	196-201
Number of pages	6
Journal	Biology Bulletin
Volume	28
Issue number	2
DOIs	https://doi.org/10.1023/A:1009479319111
State	Published - 2001

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences

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@article{77dcca1b9b004d25af6ff2b4a277fb81,

title = "DNA from various tissues of far eastern larches and its applicability for RAPD assay",

abstract = "We studied applicability of RAPD assay for investigating genetic variability of far eastern larches. Techniques to isolate DNA from larch vegetative organs (needles, young shoots and stems) and megagametophytes were selected, and conditions of polymerase chain reaction were optimized. Fifty out of 84 random primers proved efficient, and 22 and 12 primers initiated weak and no synthesis, respectively. The tested primers worked efficiently on the DNA isolated from both diploid and haploid tissues.",

author = "Sazonova, {I. Yu} and Kozyrenko, {M. M.} and Artyukova, {E. V.} and Reunova, {G. D.} and Zhuravlev, {Y. N.}",

note = "Funding Information: ACKNOWLEDGMENTS This work was supported by the Biotic Service and Inventories Program of the National Science Foundation of USA (project Dep-9505031, Theodor W. Pietsch, principal investigator) and State Research Program Biodiversity.",

year = "2001",

doi = "10.1023/A:1009479319111",

language = "English (US)",

volume = "28",

pages = "196--201",

journal = "Biology Bulletin",

issn = "1062-3590",

publisher = "Maik Nauka-Interperiodica Publishing",

number = "2",

}

TY - JOUR

T1 - DNA from various tissues of far eastern larches and its applicability for RAPD assay

AU - Sazonova, I. Yu

AU - Kozyrenko, M. M.

AU - Artyukova, E. V.

AU - Reunova, G. D.

AU - Zhuravlev, Y. N.

N1 - Funding Information: ACKNOWLEDGMENTS This work was supported by the Biotic Service and Inventories Program of the National Science Foundation of USA (project Dep-9505031, Theodor W. Pietsch, principal investigator) and State Research Program Biodiversity.

PY - 2001

Y1 - 2001

N2 - We studied applicability of RAPD assay for investigating genetic variability of far eastern larches. Techniques to isolate DNA from larch vegetative organs (needles, young shoots and stems) and megagametophytes were selected, and conditions of polymerase chain reaction were optimized. Fifty out of 84 random primers proved efficient, and 22 and 12 primers initiated weak and no synthesis, respectively. The tested primers worked efficiently on the DNA isolated from both diploid and haploid tissues.

AB - We studied applicability of RAPD assay for investigating genetic variability of far eastern larches. Techniques to isolate DNA from larch vegetative organs (needles, young shoots and stems) and megagametophytes were selected, and conditions of polymerase chain reaction were optimized. Fifty out of 84 random primers proved efficient, and 22 and 12 primers initiated weak and no synthesis, respectively. The tested primers worked efficiently on the DNA isolated from both diploid and haploid tissues.

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JF - Biology Bulletin

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ER -

DNA from various tissues of far eastern larches and its applicability for RAPD assay

Abstract

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