TY - JOUR
T1 - Down-regulation of the expression of O-acetyl-GD3 by the O- acetylesterase cDNA in hamster melanoma cells
T2 - Effects on cellular proliferation, differentiation, and melanogenesis
AU - Birklé, Stéphane
AU - Ren, Shulin
AU - Slominski, Andrzej
AU - Zeng, Guichao
AU - Gao, Luoyi
AU - Yu, Robert K.
PY - 1999
Y1 - 1999
N2 - The composition of the gangliosides of hamster melanoma cells is closely related to their cellular growth and degree of differentiation, with slow- growing, highly differentiated melanotic melanoma MI cells expressing GM3 and fast-growing, undifferentiated amelanotic Ab melanoma cells having a preponderance of GD3 and O-acetyl-GD3. To study the putative function of O- acetyl-GD3, we established stably transfected AbC-1 amelanotic hamster melanoma cells with O-acetylesterase gene from influenza C virus to hydrolyze the O-acetyl group from O-acetyl-GD3. The content of O-acetyl-GD3 in the transfected cells expressing O-acetylesterase gene was reduced by ≥90%. These O-acetyl-GD3-depleted cells differed from the parental ones in their cellular morphology, growth behavior, and melanogenesis activity. The absence of O-acetyl-GD3 in the transfected cells was accompanied by increased thick dendrite formation with an enlarged cell body, which is in striking contrast to the control cells, which were rounded and flattened, with few processes. Their growth was significantly slower than that of the control cells. They also demonstrated significantly lower tyrosinase activity and melanogenic potential. We suggest that the enhanced expression of melanoma-associated O- acetyl-GD3 ganglioside may stimulate cellular growth and suppress certain differentiated phenotypes such as dendrite formation but not melanogenesis.
AB - The composition of the gangliosides of hamster melanoma cells is closely related to their cellular growth and degree of differentiation, with slow- growing, highly differentiated melanotic melanoma MI cells expressing GM3 and fast-growing, undifferentiated amelanotic Ab melanoma cells having a preponderance of GD3 and O-acetyl-GD3. To study the putative function of O- acetyl-GD3, we established stably transfected AbC-1 amelanotic hamster melanoma cells with O-acetylesterase gene from influenza C virus to hydrolyze the O-acetyl group from O-acetyl-GD3. The content of O-acetyl-GD3 in the transfected cells expressing O-acetylesterase gene was reduced by ≥90%. These O-acetyl-GD3-depleted cells differed from the parental ones in their cellular morphology, growth behavior, and melanogenesis activity. The absence of O-acetyl-GD3 in the transfected cells was accompanied by increased thick dendrite formation with an enlarged cell body, which is in striking contrast to the control cells, which were rounded and flattened, with few processes. Their growth was significantly slower than that of the control cells. They also demonstrated significantly lower tyrosinase activity and melanogenic potential. We suggest that the enhanced expression of melanoma-associated O- acetyl-GD3 ganglioside may stimulate cellular growth and suppress certain differentiated phenotypes such as dendrite formation but not melanogenesis.
KW - Cell differentiation
KW - Cell proliferation
KW - Melanogenesis
KW - Melanoma
KW - O-Acetyl-GD3
KW - O-Acetylesterase
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U2 - 10.1046/j.1471-4159.1999.0720954.x
DO - 10.1046/j.1471-4159.1999.0720954.x
M3 - Article
C2 - 10037466
AN - SCOPUS:0033049687
SN - 0022-3042
VL - 72
SP - 954
EP - 961
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 3
ER -