Abstract
Oxidative stress has been implicated in the pathogenesis of Huntington's disease (HD), however, the origin of the oxidative stress is unknown. System xc- plays a role in the import of cystine to synthesize the antioxidant glutathione. We found in the STHdhQ7/Q7 and STHdhQ111/Q111 striatal cell lines, derived from neuronal precursor cells isolated from knock-in mice containing 7 or 111 CAG repeats in the huntingtin gene, that there is a decrease in system xc- function. System xc- is composed of two proteins, the substrate specific transporter, xCT, and an anchoring protein, CD98. The decrease in function in system xc- that we observed is caused by a decrease in xCT mRNA and protein expression in the STHdh Q111/Q111 cells. In addition, we found a decrease in protein and mRNA expression in the transgenic R6/2 HD mouse model at 6 weeks of age. STHdh Q111/Q111 cells have lower basal levels of GSH and higher basal levels of ROS. Acute inhibition of system xc- causes greater increase in oxidative stress in the STHdhQ111/Q111 cells than in the STHdhQ7/Q7 cells. These results suggest that a defect in the regulation of xCT may be involved in the pathogenesis of HD by compromising xCT expression and increasing susceptibility to oxidative stress.
Original language | English (US) |
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Pages (from-to) | 59-69 |
Number of pages | 11 |
Journal | Neurochemistry International |
Volume | 76 |
DOIs | |
State | Published - Oct 2014 |
Keywords
- Glutamate uptake
- Glutathione
- Huntington's disease
- Oxidative stress
- STHdh cells
- xCT
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
- Cell Biology