Effect of an ethanol cross-linker on universal adhesive

Allegra Comba, Tatjana Maravić, Virginia Villalta, Serena Tozzola, Claudia Mazzitelli, Vittorio Checchi, Edoardo Mancuso, Nicola Scotti, Franklin R. Tay, Lorenzo Breschi, Annalisa Mazzoni

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Objective: To evaluate the effects of N,N'-dicyclohexylcarbodiimide (DCC), an ethanol-based dentin cross-linker, on the immediate and long-term microtensile bond strength (μTBS) and nanoleakage expression of a universal adhesive employed in self-etch mode (SE) or etch-and-rinse mode (ER). The effect of DCC on the dentinal MMP activity was also investigated by means of in-situ zymography. Methods: Eighty freshly extracted human molars were sectioned to expose mid-coronal dentin surfaces. The teeth were assigned to one of the following groups, according to the dentin surface priming/adhesive approach: (G1): DCC pre-treatment and Scotchbond Universal (SBU) in ER mode; (G2): SBU in ER mode; (G3): DCC pretreatment and SBU in SE mode; (G4): SBU in SE mode. μTBS test was performed immediately (T0) or after 1-year aging (T12) in artificial saliva. Ten additional teeth per group were prepared for nanoleakage evaluation (N = 5) and for in-situ zymography (N = 5). Results: Three-factor analysis of variance revealed significant difference for the variables DCC pretreatment, application mode and aging (p < 0.05) for both microtensile bond strength testing and in-situ zymography. Nanoleakage analysis revealed reduced marginal infiltration of DCC experimental groups both at T0 and T12. Significance: The use of an ethanol-based primer containing DCC appears to be promising in preserving the stability of the adhesive interface of a universal adhesive, especially in the SE mode.

Original languageEnglish (US)
Pages (from-to)1645-1654
Number of pages10
JournalDental Materials
Issue number12
StatePublished - Dec 2020


  • Cross-Linkers
  • Dentin bonding systems
  • Matrix metalloproteinases
  • N,N'-dicyclohexylcarbodiimide
  • Universal adhesives

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials


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