Effect of carbodiimide on the fatigue crack growth resistance of resin-dentin bonds

Zihou Zhang, Dylan Beitzel, Hessam Majd, Mustafa Mutluay, Arzu Tezvergil-Mutluay, Franklin R. Tay, David H. Pashley, Dwayne Arola

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Recent studies have shown that ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) inactivates endogenous dentin proteases, thereby preventing collagen degradation and improving the durability of adhesive bonds to dentin. Bond durability is routinely assessed by monotonic microtensile testing, which does not consider the cyclic nature of mastication. Objective To characterize the effect of an EDC pretreatment on the fatigue crack growth behavior of resin-dentin bonds. Methods Bonded interface Compact Tension (CT) specimens were prepared using a three-step etch-and-rinse adhesive and hybrid resin-composite. Adhesive bonding of the treated groups included a 1 min application of an experimental EDC conditioner to the acid-etched dentin. The control groups did not receive EDC treatment. The fatigue crack growth resistance was examined after storage in artificial saliva for 0, 3 and 6 months. Results There was no significant difference in the immediate fatigue crack growth resistance of the EDC-treated and control groups at 0 months. However, after the 3 and 6 months storage periods the EDC-treated groups exhibited significantly greater (p ≤ 0.05) fatigue crack growth resistance than the control specimens. Significance Although the EDC treatment maintained the fatigue crack growth resistance of the dentin bonds through 6 months of storage, additional studies are needed to assess its effectiveness over longer periods and in relation to other cross-linking agents.

Original languageEnglish (US)
Pages (from-to)211-222
Number of pages12
JournalDental Materials
Issue number2
StatePublished - Feb 1 2016


  • Collagen
  • Cross-linker
  • Dentin bonding agents
  • Durability
  • EDC
  • Endogenous proteinases
  • Fatigue crack growth
  • Fracture

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials


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