Effect of carboidiimide on thermal denaturation temperature of dentin collagen

Milena Cadenaro; Luca Fontanive; Chiara Ottavia Navarra; Pietro Gobbi; Annalisa Mazzoni; Roberto Di Lenarda; Franklin R. Tay; David H. Pashley; Lorenzo Breschi

doi:10.1016/j.dental.2015.12.006

Effect of carboidiimide on thermal denaturation temperature of dentin collagen

Milena Cadenaro, Luca Fontanive, Chiara Ottavia Navarra, Pietro Gobbi, Annalisa Mazzoni, Roberto Di Lenarda, Franklin R. Tay, David H. Pashley, Lorenzo Breschi

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Objectives 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) has been shown to cross-link dentin type I collagen. Increased cross-linking usually elevates the glass transition temperature of polymers. The aim of this study was to evaluate the cross-linking reaction promoted by EDC in different aqueous concentrations by measuring the thermal denaturation temperature (T_d) of human dentin collagen. Methods The T_d of dehydrated collagen and of insoluble dentin matrix collagen immersed in 0.5 M or 1 M EDC aqueous solution for different treatment times was obtained using a Differential Scanning Calorimeter (DSC). Specimens were also analyzed by Energy Dispersive X-Ray Spectroscopy. Results EDC-treated dentin collagen showed a significantly higher T_d than the untreated specimens when immersed in either 0.5 M EDC or 1 M EDC for 10 min or longer (p < 0.05). EDC-treated dentin collagen showed an increase of sulfur and chloride, not detectable in EDC-untreated dentin specimens. Conversely, the relative amount of carbon, nitrogen and oxygen was not modified by treatments. Significance EDC-treated dentin collagen showed a higher T_d than the untreated control at all tested concentrations and immersion times. A higher T_d can be considered an indirect indicator of a more resistant and highly cross-linked collagen network. More data are needed to confirm these results.

Original language	English (US)
Pages (from-to)	492-498
Number of pages	7
Journal	Dental Materials
Volume	32
Issue number	4
DOIs	https://doi.org/10.1016/j.dental.2015.12.006
State	Published - Apr 1 2016

Keywords

Collagen
Cross-linking
Differential scanning calorimetry
EDC
Thermal denaturation

ASJC Scopus subject areas

General Materials Science
General Dentistry
Mechanics of Materials

Access to Document

10.1016/j.dental.2015.12.006

Cite this

@article{dec3c080e93447878d5deecefb90a933,

title = "Effect of carboidiimide on thermal denaturation temperature of dentin collagen",

abstract = "Objectives 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) has been shown to cross-link dentin type I collagen. Increased cross-linking usually elevates the glass transition temperature of polymers. The aim of this study was to evaluate the cross-linking reaction promoted by EDC in different aqueous concentrations by measuring the thermal denaturation temperature (Td) of human dentin collagen. Methods The Td of dehydrated collagen and of insoluble dentin matrix collagen immersed in 0.5 M or 1 M EDC aqueous solution for different treatment times was obtained using a Differential Scanning Calorimeter (DSC). Specimens were also analyzed by Energy Dispersive X-Ray Spectroscopy. Results EDC-treated dentin collagen showed a significantly higher Td than the untreated specimens when immersed in either 0.5 M EDC or 1 M EDC for 10 min or longer (p < 0.05). EDC-treated dentin collagen showed an increase of sulfur and chloride, not detectable in EDC-untreated dentin specimens. Conversely, the relative amount of carbon, nitrogen and oxygen was not modified by treatments. Significance EDC-treated dentin collagen showed a higher Td than the untreated control at all tested concentrations and immersion times. A higher Td can be considered an indirect indicator of a more resistant and highly cross-linked collagen network. More data are needed to confirm these results.",

keywords = "Collagen, Cross-linking, Differential scanning calorimetry, EDC, Thermal denaturation",

author = "Milena Cadenaro and Luca Fontanive and Navarra, {Chiara Ottavia} and Pietro Gobbi and Annalisa Mazzoni and {Di Lenarda}, Roberto and Tay, {Franklin R.} and Pashley, {David H.} and Lorenzo Breschi",

note = "Publisher Copyright: {\textcopyright} 2015 Academy of Dental Materials.",

year = "2016",

month = apr,

day = "1",

doi = "10.1016/j.dental.2015.12.006",

language = "English (US)",

volume = "32",

pages = "492--498",

journal = "Dental Materials",

issn = "0109-5641",

publisher = "Elsevier Science",

number = "4",

}

TY - JOUR

T1 - Effect of carboidiimide on thermal denaturation temperature of dentin collagen

AU - Cadenaro, Milena

AU - Fontanive, Luca

AU - Navarra, Chiara Ottavia

AU - Gobbi, Pietro

AU - Mazzoni, Annalisa

AU - Di Lenarda, Roberto

AU - Tay, Franklin R.

AU - Pashley, David H.

AU - Breschi, Lorenzo

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Objectives 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) has been shown to cross-link dentin type I collagen. Increased cross-linking usually elevates the glass transition temperature of polymers. The aim of this study was to evaluate the cross-linking reaction promoted by EDC in different aqueous concentrations by measuring the thermal denaturation temperature (Td) of human dentin collagen. Methods The Td of dehydrated collagen and of insoluble dentin matrix collagen immersed in 0.5 M or 1 M EDC aqueous solution for different treatment times was obtained using a Differential Scanning Calorimeter (DSC). Specimens were also analyzed by Energy Dispersive X-Ray Spectroscopy. Results EDC-treated dentin collagen showed a significantly higher Td than the untreated specimens when immersed in either 0.5 M EDC or 1 M EDC for 10 min or longer (p < 0.05). EDC-treated dentin collagen showed an increase of sulfur and chloride, not detectable in EDC-untreated dentin specimens. Conversely, the relative amount of carbon, nitrogen and oxygen was not modified by treatments. Significance EDC-treated dentin collagen showed a higher Td than the untreated control at all tested concentrations and immersion times. A higher Td can be considered an indirect indicator of a more resistant and highly cross-linked collagen network. More data are needed to confirm these results.

AB - Objectives 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) has been shown to cross-link dentin type I collagen. Increased cross-linking usually elevates the glass transition temperature of polymers. The aim of this study was to evaluate the cross-linking reaction promoted by EDC in different aqueous concentrations by measuring the thermal denaturation temperature (Td) of human dentin collagen. Methods The Td of dehydrated collagen and of insoluble dentin matrix collagen immersed in 0.5 M or 1 M EDC aqueous solution for different treatment times was obtained using a Differential Scanning Calorimeter (DSC). Specimens were also analyzed by Energy Dispersive X-Ray Spectroscopy. Results EDC-treated dentin collagen showed a significantly higher Td than the untreated specimens when immersed in either 0.5 M EDC or 1 M EDC for 10 min or longer (p < 0.05). EDC-treated dentin collagen showed an increase of sulfur and chloride, not detectable in EDC-untreated dentin specimens. Conversely, the relative amount of carbon, nitrogen and oxygen was not modified by treatments. Significance EDC-treated dentin collagen showed a higher Td than the untreated control at all tested concentrations and immersion times. A higher Td can be considered an indirect indicator of a more resistant and highly cross-linked collagen network. More data are needed to confirm these results.

KW - Collagen

KW - Cross-linking

KW - Differential scanning calorimetry

KW - EDC

KW - Thermal denaturation

UR - http://www.scopus.com/inward/record.url?scp=84952927364&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84952927364&partnerID=8YFLogxK

U2 - 10.1016/j.dental.2015.12.006

DO - 10.1016/j.dental.2015.12.006

M3 - Article

C2 - 26764172

AN - SCOPUS:84952927364

SN - 0109-5641

VL - 32

SP - 492

EP - 498

JO - Dental Materials

JF - Dental Materials

IS - 4

ER -

Effect of carboidiimide on thermal denaturation temperature of dentin collagen

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this