Effect of chitosan as a cross-linker on matrix metalloproteinase activity and bond stability with different adhesive systems

Eugenia Baena, Sandra R. Cunha, Tatjana Maravić, Allegra Comba, Federica Paganelli, Giulio Alessandri-Bonetti, Laura Ceballos, Franklin R. Tay, Lorenzo Breschi, Annalisa Mazzoni

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


The aim of the present study was to evaluate the effect of 0.1% chitosan (Ch) solution as an additional primer on the mechanical durability and enzymatic activity on dentine using an etch-and-rinse (E&R) adhesive and a universal self-etch (SE) adhesive. Microtensile bond strength and interfacial nanoleakage expression of the bonded interfaces for all adhesives (with or without pretreatment with 0.1% Ch solution for 1 min and air-dried for 5 s) were analyzed immediately and after 10,000 thermocycles. Zymograms of protein extracts from human dentine powder incubated with Optibond FL and Scotchbond Universal on untreated or Ch-treated dentine were obtained to examine dentine matrix metalloproteinase (MMP) activities. The use of 0.1% Ch solution as an additional primer in conjunction with the E&R or SE adhesive did not appear to have influenced the immediate bond strength (T0) or bond strength after thermocycling (T1). Zymography showed a reduction in MMP activities only for mineralized and demineralized dentine powder after the application of Ch. Application of 0.1% Ch solution does not increase the longevity of resin-dentine bonds. Nonetheless, the procedure appears to be proficient in reducing dentine MMP activities within groups without adhesive treatments. Further studies are required to comprehend the cross-linking of Ch with dentine collagen.

Original languageEnglish (US)
Article number263
JournalMarine Drugs
Issue number5
StatePublished - May 2020


  • Bond strength
  • Chitosan
  • Cross-linkers
  • Matrix metalloproteinases
  • Zymography

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery
  • Pharmacology, Toxicology and Pharmaceutics (miscellaneous)


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