TY - JOUR
T1 - Effect of UVA-activated riboflavin on dentin bonding
AU - Cova, A.
AU - Breschi, L.
AU - Nato, F.
AU - Ruggeri, A.
AU - Carrilho, M.
AU - Tjäderhane, L.
AU - Prati, C.
AU - Di Lenarda, R.
AU - Tay, Franklin Chi Meng
AU - Pashley, David Henry
AU - Mazzoni, A.
N1 - Funding Information:
The study was funded with an FIRB grant (MIUR, Italy), and by grants R21 DE 091213 (P.I. FRT) and R01 DE 015306-07 from the NIH/NIDCR (P.I. DHP).
PY - 2011/12
Y1 - 2011/12
N2 - Recent studies have reported collagen cross-linking after exposure to riboflavin followed by ultraviolet-A (UVA) exposure. This study is the first to investigate the effect of a riboflavin-containing primer on adhesive interface stability and dentinal matrix metalloproteinase activity. Human dentin was etched with 35% phosphoric acid, treated with 0.1% riboflavin, exposed to UVA for 2 min, and bonded with a two-step etch-and-rinse adhesive. Adhesive was applied to control specimens without riboflavin/UVA. Specimens were subjected to microtensile bond strength tests and pulled to failure after storage for 24 hrs, 6 mos, or 1 yr. Interfacial nanoleakage was evaluated by light and transmission electron microscopy. To investigate dentinal matrix metalloproteinase activity, we performed correlative zymographic assays on protein extracts obtained from phosphoric-acid-etched dentin powder with or without riboflavin/UVA treatment and XP Bond. Ultraviolet-activated riboflavin treatment increased the immediate bond strength to dentin at all aging intervals (p < 0.05 vs. control) and decreased interfacial nanoleakage in aged specimens (1 yr; p < 0.05). Zymograms revealed that riboflavin/UVA pre-treatment inhibited dentinal matrix metalloproteinase activity (especially MMP-9). In conclusion, dentinal collagen cross-linking induced by riboflavin/UVA increased immediate bond strength, stabilized the adhesive interface, and inhibited dentin matrix metalloproteinases, thereby increasing the durability of resin-dentin bonds.
AB - Recent studies have reported collagen cross-linking after exposure to riboflavin followed by ultraviolet-A (UVA) exposure. This study is the first to investigate the effect of a riboflavin-containing primer on adhesive interface stability and dentinal matrix metalloproteinase activity. Human dentin was etched with 35% phosphoric acid, treated with 0.1% riboflavin, exposed to UVA for 2 min, and bonded with a two-step etch-and-rinse adhesive. Adhesive was applied to control specimens without riboflavin/UVA. Specimens were subjected to microtensile bond strength tests and pulled to failure after storage for 24 hrs, 6 mos, or 1 yr. Interfacial nanoleakage was evaluated by light and transmission electron microscopy. To investigate dentinal matrix metalloproteinase activity, we performed correlative zymographic assays on protein extracts obtained from phosphoric-acid-etched dentin powder with or without riboflavin/UVA treatment and XP Bond. Ultraviolet-activated riboflavin treatment increased the immediate bond strength to dentin at all aging intervals (p < 0.05 vs. control) and decreased interfacial nanoleakage in aged specimens (1 yr; p < 0.05). Zymograms revealed that riboflavin/UVA pre-treatment inhibited dentinal matrix metalloproteinase activity (especially MMP-9). In conclusion, dentinal collagen cross-linking induced by riboflavin/UVA increased immediate bond strength, stabilized the adhesive interface, and inhibited dentin matrix metalloproteinases, thereby increasing the durability of resin-dentin bonds.
KW - collagen cross-linking
KW - dentin bonding agent
KW - dentin matrix
KW - matrix metalloproteinases
KW - nanoleakage
KW - riboflavin
UR - http://www.scopus.com/inward/record.url?scp=81555203195&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=81555203195&partnerID=8YFLogxK
U2 - 10.1177/0022034511423397
DO - 10.1177/0022034511423397
M3 - Article
C2 - 21940521
AN - SCOPUS:81555203195
SN - 0022-0345
VL - 90
SP - 1439
EP - 1445
JO - Journal of Dental Research
JF - Journal of Dental Research
IS - 12
ER -