TY - JOUR
T1 - Effect of vitamin E and eccentric exercise on selected biomarkers of oxidative stress in young and elderly men
AU - Sacheck, Jennifer M.
AU - Milbury, Paul E.
AU - Cannon, Joseph G.
AU - Roubenoff, Ronenn
AU - Blumberg, Jeffrey B.
N1 - Funding Information:
Support was provided by the Saga Research Institute of Otsuka Pharmaceutical Co., Ltd., Kanzaki, Japan; Pharmavite, Inc., San Fernando, CA; U.S. Department of Agriculture (USDA) Agricultural Research Service under Cooperative Agreement No. 58-1950-001; and the American College of Sports Medicine and LifeFitness. We appreciate the contributions made to this study by the staff of the Metabolic Research Unit and Nutrition Evaluation Laboratory at the HNRCA at Tufts. We thank Dr. Wayne Matson of ESA, Inc. for the generous contribution of his time and expertise and access to instrumentation for the determination of 8-OHdG. We also thank Dr. Mary Walter for her help in the analysis of iPF 2α .
PY - 2003/6/15
Y1 - 2003/6/15
N2 - Muscle damage resulting from eccentric exercise provides a useful model of oxyradical-induced injury and can be used to examine age-related responses to oxidative stress. Sixteen young (26.4 ± 3.3 years) and 16 older (71.1 ± 4.0 years) healthy men were randomly assigned to 1000 IU/d vitamin E or placebo for 12 weeks and ran downhill for 45 min at 75% VO2max, once before and following supplementation. Blood samples were obtained before (baseline) and immediately postexercise (0 h), and at 6, 24, and 72 h postexercise to determine antioxidant status, muscle damage, lipid peroxidation, and DNA damage. Following exercise, young and older men experienced similar increases in serum creatine kinase (CK), F2α-isoprostanes (iPF2α; p < .001) and malondialdehyde (MDA; p < .01), although iPF2α peaked at 72 h postexercise and MDA peaked at 0 h. Oxygen Radical Absorbance Capacity (ORAC) decreased at 72 h (p < .01) and correlated with the rise in iPF2α, MDA, and CK in the young men (p < .05). Leukocyte 8-hydroxy-2′-deoxyguanosine (8-OHdG) was unaffected by exercise. Vitamin E decreased peak CK in young men, while in older men it decreased resting levels of iPF2α and suppressed the 24 h postexercise increases in iPF2α (p < .05). Thus, vitamin E supplementation induced modest changes eccentric exercise-induced oxidative stress, although differentially between the young and older subjects, while age had no direct influence on these responses among this group of physically fit subjects.
AB - Muscle damage resulting from eccentric exercise provides a useful model of oxyradical-induced injury and can be used to examine age-related responses to oxidative stress. Sixteen young (26.4 ± 3.3 years) and 16 older (71.1 ± 4.0 years) healthy men were randomly assigned to 1000 IU/d vitamin E or placebo for 12 weeks and ran downhill for 45 min at 75% VO2max, once before and following supplementation. Blood samples were obtained before (baseline) and immediately postexercise (0 h), and at 6, 24, and 72 h postexercise to determine antioxidant status, muscle damage, lipid peroxidation, and DNA damage. Following exercise, young and older men experienced similar increases in serum creatine kinase (CK), F2α-isoprostanes (iPF2α; p < .001) and malondialdehyde (MDA; p < .01), although iPF2α peaked at 72 h postexercise and MDA peaked at 0 h. Oxygen Radical Absorbance Capacity (ORAC) decreased at 72 h (p < .01) and correlated with the rise in iPF2α, MDA, and CK in the young men (p < .05). Leukocyte 8-hydroxy-2′-deoxyguanosine (8-OHdG) was unaffected by exercise. Vitamin E decreased peak CK in young men, while in older men it decreased resting levels of iPF2α and suppressed the 24 h postexercise increases in iPF2α (p < .05). Thus, vitamin E supplementation induced modest changes eccentric exercise-induced oxidative stress, although differentially between the young and older subjects, while age had no direct influence on these responses among this group of physically fit subjects.
KW - Aging
KW - Antioxidants
KW - DNA damage
KW - Exercise
KW - Free radicals
KW - Lipid peroxidation
KW - Muscle damage
KW - Vitamin E
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U2 - 10.1016/S0891-5849(03)00187-4
DO - 10.1016/S0891-5849(03)00187-4
M3 - Article
C2 - 12788477
AN - SCOPUS:0037683669
SN - 0891-5849
VL - 34
SP - 1575
EP - 1588
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
IS - 12
ER -