Effect of vitamin E and eccentric exercise on selected biomarkers of oxidative stress in young and elderly men

Jennifer M. Sacheck, Paul E. Milbury, Joseph G. Cannon, Ronenn Roubenoff, Jeffrey B. Blumberg

Research output: Contribution to journalArticlepeer-review

203 Scopus citations


Muscle damage resulting from eccentric exercise provides a useful model of oxyradical-induced injury and can be used to examine age-related responses to oxidative stress. Sixteen young (26.4 ± 3.3 years) and 16 older (71.1 ± 4.0 years) healthy men were randomly assigned to 1000 IU/d vitamin E or placebo for 12 weeks and ran downhill for 45 min at 75% VO2max, once before and following supplementation. Blood samples were obtained before (baseline) and immediately postexercise (0 h), and at 6, 24, and 72 h postexercise to determine antioxidant status, muscle damage, lipid peroxidation, and DNA damage. Following exercise, young and older men experienced similar increases in serum creatine kinase (CK), F-isoprostanes (iPF; p < .001) and malondialdehyde (MDA; p < .01), although iPF peaked at 72 h postexercise and MDA peaked at 0 h. Oxygen Radical Absorbance Capacity (ORAC) decreased at 72 h (p < .01) and correlated with the rise in iPF, MDA, and CK in the young men (p < .05). Leukocyte 8-hydroxy-2′-deoxyguanosine (8-OHdG) was unaffected by exercise. Vitamin E decreased peak CK in young men, while in older men it decreased resting levels of iPF and suppressed the 24 h postexercise increases in iPF (p < .05). Thus, vitamin E supplementation induced modest changes eccentric exercise-induced oxidative stress, although differentially between the young and older subjects, while age had no direct influence on these responses among this group of physically fit subjects.

Original languageEnglish (US)
Pages (from-to)1575-1588
Number of pages14
JournalFree Radical Biology and Medicine
Issue number12
StatePublished - Jun 15 2003


  • Aging
  • Antioxidants
  • DNA damage
  • Exercise
  • Free radicals
  • Lipid peroxidation
  • Muscle damage
  • Vitamin E

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)


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