Effect of water-aging on the antimicrobial activities of an ORMOSIL-containing orthodontic acrylic resin

Shi Qiang Gong; D. Jeevanie Epasinghe; Bin Zhou; Li Na Niu; Kirk A. Kimmerling; Frederick A. Rueggeberg; Cynthia K.Y. Yiu; Jing Mao; David H. Pashley; Franklin R. Tay

doi:10.1016/j.actbio.2013.02.031

Effect of water-aging on the antimicrobial activities of an ORMOSIL-containing orthodontic acrylic resin

Shi Qiang Gong, D. Jeevanie Epasinghe, Bin Zhou, Li Na Niu, Kirk A. Kimmerling, Frederick A. Rueggeberg, Cynthia K.Y. Yiu, Jing Mao, David H. Pashley, Franklin R. Tay

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Quaternary ammonium methacryloxy silicate (QAMS), an organically modified silicate (ORMOSIL) functionalized with polymerizable methacrylate groups and an antimicrobial agent with a long lipophilic alkyl chain quaternary ammonium group, was synthesized through a silane-based sol-gel route. By dissolving QAMS in methyl methacrylate monomer, this ORMOSIL molecule was incorporated into an auto-polymerizing, powder/liquid orthodontic acrylic resin system, yielding QAMS-containing poly(methyl methacrylate). The QAMS-containing acrylic resin showed a predominant contact-killing effect on Streptococcus mutans (ATCC 35668) and Actinomyces naeslundii (ATCC 12104) biofilms, while inhibiting adhesion of Candida albicans (ATCC 90028) on the acrylic surface. The antimicrobial activities of QAMS-containing acrylic resin were maintained after a 3 month water-aging period. Bromophenol blue assay showed minimal leaching of quaternary ammonium species when an appropriate amount of QAMS (<4 wt.%) was incorporated into the acrylic resin. The results suggest that QAMS is predominantly co-polymerized with the poly(methyl methacrylate) network, and only a minuscule amount of free QAMS molecules is present within the polymer network after water-aging. Acrylic resin with persistent antimicrobial activities represents a promising method for preventing bacteria- and fungus-induced stomatitis, an infectious disease commonly associated with the wearing of removable orthodontic appliances.

Original language	English (US)
Pages (from-to)	6964-6973
Number of pages	10
Journal	Acta biomaterialia
Volume	9
Issue number	6
DOIs	https://doi.org/10.1016/j.actbio.2013.02.031
State	Published - Jun 2013

Keywords

Antimicrobial
Biofilm
Ormosil
Orthodontic base polymer
Polymethyl methacrylate

ASJC Scopus subject areas

Biotechnology
Biomaterials
Biochemistry
Biomedical Engineering
Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.actbio.2013.02.031

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@article{a685eff6adc8485fbeef84e814331d09,

title = "Effect of water-aging on the antimicrobial activities of an ORMOSIL-containing orthodontic acrylic resin",

abstract = "Quaternary ammonium methacryloxy silicate (QAMS), an organically modified silicate (ORMOSIL) functionalized with polymerizable methacrylate groups and an antimicrobial agent with a long lipophilic alkyl chain quaternary ammonium group, was synthesized through a silane-based sol-gel route. By dissolving QAMS in methyl methacrylate monomer, this ORMOSIL molecule was incorporated into an auto-polymerizing, powder/liquid orthodontic acrylic resin system, yielding QAMS-containing poly(methyl methacrylate). The QAMS-containing acrylic resin showed a predominant contact-killing effect on Streptococcus mutans (ATCC 35668) and Actinomyces naeslundii (ATCC 12104) biofilms, while inhibiting adhesion of Candida albicans (ATCC 90028) on the acrylic surface. The antimicrobial activities of QAMS-containing acrylic resin were maintained after a 3 month water-aging period. Bromophenol blue assay showed minimal leaching of quaternary ammonium species when an appropriate amount of QAMS (<4 wt.%) was incorporated into the acrylic resin. The results suggest that QAMS is predominantly co-polymerized with the poly(methyl methacrylate) network, and only a minuscule amount of free QAMS molecules is present within the polymer network after water-aging. Acrylic resin with persistent antimicrobial activities represents a promising method for preventing bacteria- and fungus-induced stomatitis, an infectious disease commonly associated with the wearing of removable orthodontic appliances.",

keywords = "Antimicrobial, Biofilm, Ormosil, Orthodontic base polymer, Polymethyl methacrylate",

author = "Gong, {Shi Qiang} and Epasinghe, {D. Jeevanie} and Bin Zhou and Niu, {Li Na} and Kimmerling, {Kirk A.} and Rueggeberg, {Frederick A.} and Yiu, {Cynthia K.Y.} and Jing Mao and Pashley, {David H.} and Tay, {Franklin R.}",

note = "Funding Information: This work was supported by grant R01 DE015306-06 from NIDCR (PI. David Pashley), IRRM award from Georgia Health Sciences University (PI. Franklin Tay), and General Research Fund (GRF) HKU 784410 M from The University of Hong Kong (PI. Cynthia Yiu). The authors thank Mrs Michelle Barnes for her secretarial support. ",

year = "2013",

month = jun,

doi = "10.1016/j.actbio.2013.02.031",

language = "English (US)",

volume = "9",

pages = "6964--6973",

journal = "Acta biomaterialia",

issn = "1742-7061",

publisher = "Elsevier BV",

number = "6",

}

TY - JOUR

T1 - Effect of water-aging on the antimicrobial activities of an ORMOSIL-containing orthodontic acrylic resin

AU - Gong, Shi Qiang

AU - Epasinghe, D. Jeevanie

AU - Zhou, Bin

AU - Niu, Li Na

AU - Kimmerling, Kirk A.

AU - Rueggeberg, Frederick A.

AU - Yiu, Cynthia K.Y.

AU - Mao, Jing

AU - Pashley, David H.

AU - Tay, Franklin R.

N1 - Funding Information: This work was supported by grant R01 DE015306-06 from NIDCR (PI. David Pashley), IRRM award from Georgia Health Sciences University (PI. Franklin Tay), and General Research Fund (GRF) HKU 784410 M from The University of Hong Kong (PI. Cynthia Yiu). The authors thank Mrs Michelle Barnes for her secretarial support.

PY - 2013/6

Y1 - 2013/6

N2 - Quaternary ammonium methacryloxy silicate (QAMS), an organically modified silicate (ORMOSIL) functionalized with polymerizable methacrylate groups and an antimicrobial agent with a long lipophilic alkyl chain quaternary ammonium group, was synthesized through a silane-based sol-gel route. By dissolving QAMS in methyl methacrylate monomer, this ORMOSIL molecule was incorporated into an auto-polymerizing, powder/liquid orthodontic acrylic resin system, yielding QAMS-containing poly(methyl methacrylate). The QAMS-containing acrylic resin showed a predominant contact-killing effect on Streptococcus mutans (ATCC 35668) and Actinomyces naeslundii (ATCC 12104) biofilms, while inhibiting adhesion of Candida albicans (ATCC 90028) on the acrylic surface. The antimicrobial activities of QAMS-containing acrylic resin were maintained after a 3 month water-aging period. Bromophenol blue assay showed minimal leaching of quaternary ammonium species when an appropriate amount of QAMS (<4 wt.%) was incorporated into the acrylic resin. The results suggest that QAMS is predominantly co-polymerized with the poly(methyl methacrylate) network, and only a minuscule amount of free QAMS molecules is present within the polymer network after water-aging. Acrylic resin with persistent antimicrobial activities represents a promising method for preventing bacteria- and fungus-induced stomatitis, an infectious disease commonly associated with the wearing of removable orthodontic appliances.

AB - Quaternary ammonium methacryloxy silicate (QAMS), an organically modified silicate (ORMOSIL) functionalized with polymerizable methacrylate groups and an antimicrobial agent with a long lipophilic alkyl chain quaternary ammonium group, was synthesized through a silane-based sol-gel route. By dissolving QAMS in methyl methacrylate monomer, this ORMOSIL molecule was incorporated into an auto-polymerizing, powder/liquid orthodontic acrylic resin system, yielding QAMS-containing poly(methyl methacrylate). The QAMS-containing acrylic resin showed a predominant contact-killing effect on Streptococcus mutans (ATCC 35668) and Actinomyces naeslundii (ATCC 12104) biofilms, while inhibiting adhesion of Candida albicans (ATCC 90028) on the acrylic surface. The antimicrobial activities of QAMS-containing acrylic resin were maintained after a 3 month water-aging period. Bromophenol blue assay showed minimal leaching of quaternary ammonium species when an appropriate amount of QAMS (<4 wt.%) was incorporated into the acrylic resin. The results suggest that QAMS is predominantly co-polymerized with the poly(methyl methacrylate) network, and only a minuscule amount of free QAMS molecules is present within the polymer network after water-aging. Acrylic resin with persistent antimicrobial activities represents a promising method for preventing bacteria- and fungus-induced stomatitis, an infectious disease commonly associated with the wearing of removable orthodontic appliances.

KW - Antimicrobial

KW - Biofilm

KW - Ormosil

KW - Orthodontic base polymer

KW - Polymethyl methacrylate

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U2 - 10.1016/j.actbio.2013.02.031

DO - 10.1016/j.actbio.2013.02.031

M3 - Article

C2 - 23485857

AN - SCOPUS:84877698887

SN - 1742-7061

VL - 9

SP - 6964

EP - 6973

JO - Acta biomaterialia

JF - Acta biomaterialia

IS - 6

ER -

Effect of water-aging on the antimicrobial activities of an ORMOSIL-containing orthodontic acrylic resin

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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