Effects of an experimental calcium aluminosilicate cement on the viability of murine odontoblast-like cells

Wei Wei, Yi Pin Qi, Sergey Y. Nikonov, Li Na Niu, Regina L.W. Messer, Jing Mao, Carolyn M. Primus, David H. Pashley, Franklin R. Tay

Research output: Contribution to journalArticlepeer-review

42 Scopus citations


Introduction: Quick-setting calcium aluminosilicate cement with improved washout resistance is a potential substitute for calcium silicate cements in endodontics. This study examined the effect of an experimental calcium aluminosilicate cement (Quick-Set; Primus Consulting, Bradenton, FL) on the viability of odontoblast-like cells. Methods: The biocompatibility of Quick-Set and white ProRoot MTA (WMTA; Dentsply Tulsa Dental Specialties, Tulsa, OK) cements and their eluents was evaluated using a murine dental papilla-derived odontoblast-like cell line (MDPC-23); 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay was used to examine the effects of the 2 hydraulic cements on mitochondrial metabolic activity. Flow cytometry and confocal laser scanning microscopy were used to identify the effects of the 2 cements on cell death-induced plasma membrane permeability to fluorescent dyes and DNA stains. Results: After the first week of immersion in culture medium, Quick-Set and WMTA were more cytotoxic than the Teflon-negative control (P <.05), and the cells exhibited more apoptosis/necrosis than Teflon (P <.05). After the second week of immersion, the 2 cements were as biocompatible as Teflon (P >.05), with cells exhibiting minimal apoptosis/necrosis. Eluents from the set cements at 1:1 dilution were significantly more cytotoxic that eluents at 1:10 or 1:100 dilution (P <.05). Conclusions: Quick-Set and WMTA exhibited similar cytotoxicity profiles. They possess negligible in vitro toxicologic risks after time-dependent elution of toxic components.

Original languageEnglish (US)
Pages (from-to)936-942
Number of pages7
JournalJournal of endodontics
Issue number7
StatePublished - Jul 2012


  • 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
  • MTT assay
  • biocompatibility
  • calcium aluminate
  • flow cytometry
  • hydraulic cement
  • vital cell staining

ASJC Scopus subject areas

  • Dentistry(all)


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