Effects of etch-and-rinse and self-etch adhesives on dentin MMP-2 and MMP-9

A. Mazzoni, P. Scaffa, M. Carrilho, L. Tjäderhane, R. Di Lenarda, A. Polimeni, A. Tezvergil-Mutluay, F. R. Tay, D. H. Pashley, L. Breschi

Research output: Contribution to journalArticlepeer-review

150 Scopus citations


Auto-degradation of collagen matrices occurs within hybrid layers created by contemporary dentin bonding systems, by the slow action of host-derived matrix metalloproteinases (MMPs). This study tested the null hypothesis that there are no differences in the activities of MMP-2 and -9 after treatment with different etch-and-rinse or self-etch adhesives. Tested adhesives were: Adper Scotchbond 1XT (3M ESPE), PQ1 (Ultradent), Peak LC (Ultradent), Optibond Solo Plus (Kerr), Prime&Bond NT (Dentsply) (all 2-step etch-and-rinse adhesives), and Adper Easy Bond (3M ESPE), Tri-S (Kuraray), and Xeno-V (Dentsply) (1-step self-etch adhesives). MMP-2 and -9 activities were quantified in adhesive-treated dentin powder by means of an activity assay and gelatin zymography. MMP-2 and MMP-9 activities were found after treatment with all of the simplified etch-and-rinse and self-etch adhesives; however, the activation was adhesive-dependent. It is concluded that all two-step etch-and-rinse and the one-step self-etch adhesives tested can activate endogenous MMP-2 and MMP-9 in human dentin. These results support the role of endogenous MMPs in the degradation of hybrid layers created by these adhesives.

Original languageEnglish (US)
Pages (from-to)82-86
Number of pages5
JournalJournal of Dental Research
Issue number1
StatePublished - Jan 2013
Externally publishedYes


  • auto-degradation
  • biochemical assays
  • collagen
  • dentin bonding systems
  • hybrid layer
  • non-collageneous proteins

ASJC Scopus subject areas

  • Dentistry(all)


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